| Optimization of methods to study pulmonary dendritic cell migration reveals distinct capacities of DC subsets to acquire soluble versus particulate antigen. | |
| | |
MedLine Citation:
|
PMID: 18662693 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Dendritic cell migration from the airway to lymph nodes is a key event in the development of airway immunity during infection, allergy, and vaccination. To identify the best approaches to investigate DC migration to lung-draining lymph nodes, we directly compared three methods previously used to track DC migration: airway administration of fluorescent OVA, latex beads, or carboxyfluorescein succinimidyl ester (CFSE). We show that two of the methods employed in optimal conditions-administration of fluorescent OVA or latex particles-have broadly relevant utility in studies of pulmonary DC migration, both in the presence and absence of inflammatory mediators. However, CFSE was of limited value because it induced a robust airway inflammatory response upon instillation. Unexpectedly, antigen-loaded tracers with distinct physical properties differently affected the populations that acquired the tracers and the overall T cell response. Specifically, soluble OVA and OVA formulated as a particulate after conjugation to latex beads were acquired in different proportions in vivo by the two characterized subsets of pulmonary DCs: CD11b(hi)CD103(-) and CD11b(lo)CD103(+)langerin(+) DC populations. Consequently, and in line with recent studies that these two subsets of DCs respectively activate CD4(+) and CD8(+) lymphocyte populations, the physical nature of the antigen delivery vehicle strongly influenced the degree of CD4(+) versus CD8(+) OVA-specific T cell activation. This finding suggests that changes in the physical presentation of the same antigen delivered to the airway during natural immune responses or vaccinations may markedly affect the character of the T cell response that ensues. |
| | |
Authors:
|
Claudia Jakubzick; Julie Helft; Theodore J Kaplan; Gwendalyn J Randolph |
Related Documents
:
|
18194833 - Effects of fumonisin b(1) on the expression of cytokines and chemokines in human dendri... 16019503 - Dendritic cells from patients with chronic myeloid leukemia: functional and phenotypic ... 11110703 - Interferon alpha prevents spontaneous apoptosis of clonal th2 cells associated with chr... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-07-26 |
Journal Detail:
|
Title: Journal of immunological methods Volume: 337 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 2008 Sep |
Date Detail:
|
Created Date: 2008-08-25 Completed Date: 2008-10-07 Revised Date: 2010-12-30 |
Medline Journal Info:
|
Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 121-31 Citation Subset: IM |
Affiliation:
|
Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, NY, New York 10029, United States. claudia.jakubzick@mssm.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigen Presentation* Antigens, CD / analysis Antigens, CD11b / analysis CD8-Positive T-Lymphocytes / immunology Cell Movement Dendritic Cells / physiology* Integrin alpha Chains / analysis Lung / immunology* Lymph Nodes / immunology Lymphocyte Activation Mice Mice, Inbred C57BL Pertussis Toxin / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
AI49653/AI/NIAID NIH HHS; R01 AI061741-02/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD; 0/Antigens, CD11b; 0/Integrin alpha Chains; 0/alpha E integrins; EC 2.4.2.31/Pertussis Toxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Changes in brain seabream GnRH mRNA and pituitary seabream GnRH peptide levels during ovarian matura...
Next Document: Quantification of circulating mature endothelial cells using a whole blood four-color flow cytometri...