Document Detail


Optimization of gene delivery to HEK293T cells by microporation using a central composite design methodology.
MedLine Citation:
PMID:  20559681     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microporation is an efficient method for delivering plasmid DNA molecules into cultured cells. Herein, we present the optimization of gene delivery by microporation using a Central Composite Design methodology. It was given relevance not only to the transfection efficiency but also to the cell recovery. Different amounts of DNA (1 and 3 μg) mainly affected cell viabilities and cell recoveries, which decrease from 93 to 76% and from 47 to 25% respectively, when higher DNA quantity is used. With this work we suggest an easy methodology to improve transfection of mammalian cells underlining the feasibility to achieve 60% of gene delivery efficiencies whilst recovering 50% of cells, with 90% of viability.
Authors:
C Madeira; S C Ribeiro; M Z Turk; J M S Cabral
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-18
Journal Detail:
Title:  Biotechnology letters     Volume:  32     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-17     Completed Date:  2011-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1393-9     Citation Subset:  IM    
Affiliation:
IBB-Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisbon, Portugal. catarina.madeira@ist.utl.pt
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MeSH Terms
Descriptor/Qualifier:
Biotechnology / methods*
Cell Line
Cell Survival
Electroporation / methods*
Humans
Transfection*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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