| Optimization of gene delivery to HEK293T cells by microporation using a central composite design methodology. | |
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MedLine Citation:
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PMID: 20559681 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microporation is an efficient method for delivering plasmid DNA molecules into cultured cells. Herein, we present the optimization of gene delivery by microporation using a Central Composite Design methodology. It was given relevance not only to the transfection efficiency but also to the cell recovery. Different amounts of DNA (1 and 3 μg) mainly affected cell viabilities and cell recoveries, which decrease from 93 to 76% and from 47 to 25% respectively, when higher DNA quantity is used. With this work we suggest an easy methodology to improve transfection of mammalian cells underlining the feasibility to achieve 60% of gene delivery efficiencies whilst recovering 50% of cells, with 90% of viability. |
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Authors:
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C Madeira; S C Ribeiro; M Z Turk; J M S Cabral |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-18 |
Journal Detail:
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Title: Biotechnology letters Volume: 32 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-17 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1393-9 Citation Subset: IM |
Affiliation:
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IBB-Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisbon, Portugal. catarina.madeira@ist.utl.pt |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biotechnology
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methods* Cell Line Cell Survival Electroporation / methods* Humans Transfection* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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