Document Detail


Optimization of freezing, storage, and thawing conditions for the preparation of metabolically active synaptosomes from frozen rat and human brain.
MedLine Citation:
PMID:  3561893     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Samples of rat and human cerebral cortex were frozen, stored, and thawed under a variety of conditions to define further the optimal procedure for storing human brain samples for subsequent metabolic and functional studies that use incubated synaptosomes. Tissue samples were best preserved by immersing them in isotonic sucrose prior to slow freezing, but there was no advantage in first chopping up the material. High concentrations of sucrose, rather than exerting a cryoprotective effect, were detrimental to subsequent synaptosomal performance (oxygen uptake, K+ accumulation, stimulus-induced release of amino acid neurotransmitters). However, good activity was observed in preparations from rat brain frozen in the absence of fluid. This result was confirmed by studies on the uptake of gamma-aminobutyrate (GABA) into an osmotically sensitive compartment in synaptosomes prepared from frozen human autopsy material transshipped from the brain tissue collection ("brain bank") at Harvard Medical School, MA, USA, to Sydney, Australia. Although activity was slowly lost over a 3-mo period in rat tissue samples stored at -20 degrees C, there was little or no such loss at -70 degrees C.
Authors:
P R Dodd; J A Hardy; E B Baig; A M Kidd; E D Bird; W E Watson; G A Johnston
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemical pathology     Volume:  4     ISSN:  0734-600X     ISO Abbreviation:  Neurochem Pathol     Publication Date:  1986 Jun 
Date Detail:
Created Date:  1987-05-11     Completed Date:  1987-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8310818     Medline TA:  Neurochem Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  177-98     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cerebral Cortex / metabolism,  ultrastructure*
Female
Freezing
Humans
Male
Rats
Rats, Inbred Strains
Solutions
Sucrose
Synaptosomes* / metabolism
Tissue Preservation / methods*
gamma-Aminobutyric Acid / metabolism
Chemical
Reg. No./Substance:
0/Solutions; 56-12-2/gamma-Aminobutyric Acid; 57-50-1/Sucrose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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