Document Detail

Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology.
MedLine Citation:
PMID:  17953686     Owner:  NLM     Status:  MEDLINE    
AIMS: To evaluate the influence of environmental parameters on the production of antibiotics (xenocoumacins and nematophin) by Xenorhabdus nematophila and enhance the antibiotic activity. METHODS AND RESULTS: Response surface methodology (RSM) was employed to study the effects of five parameters (the initial pH, medium volume in flask, rotary speed, temperature and inoculation volume) on the production of antibiotics in flask cultures by X. nematophila YL001. A 2(5-1)-factorial central composite design was chosen to explain the combined effects of the five parameters and to design a minimum number of experiments. The experimental results and software-predicted values of production of antibiotics were comparable. The statistical analysis of the results showed that, in the range studied, medium volume in flask, rotary speed, temperature and inoculation volume had a significant effect (P < 0.05) on the production of antibiotics at their individual level, medium volume in flask and rotary speed showed a significant influence at interactive level and were most significant at individual level. The maximum antibiotic activity was achieved at the initial pH 7.64, medium volume in 250 ml flask 25 ml, rotary speed of 220 rev min(-1), temperature 27.8 degrees C and inoculation volume of 15.0%. Maximum antibiotic activity of 331.7 U ml(-1) was achieved under the optimized condition. CONCLUSIONS: As far as known, there are no reports of production of antibiotic from X. nematophila by engineering the condition of fermentation using RSM. The results strongly support the use of RSM for fermentation condition optimization. The optimization of the environmental parameters resulted not only in a 43.4% higher antibiotic activity than unoptimized conditions but also in a reduced amount of the experiments. The chosen method of optimization of fermentation condition was efficient, relatively simple and time and material saving. SIGNIFICANCE AND IMPACT OF THE STUDY: This study should contribute towards improving the antibiotics activity of X. nematophila. Integrated into a broader study of the impact of environmental factors on the production of antibiotic, this work should help to build more rational control strategy, possibly involving scale-up of production of antibiotics by X. nematophila.
Y-H Wang; J-T Feng; Q Zhang; X Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-22
Journal Detail:
Title:  Journal of applied microbiology     Volume:  104     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-21     Completed Date:  2008-05-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  735-44     Citation Subset:  IM    
Biorational Pesticides Research and Service Center, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, China.
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MeSH Terms
Anti-Bacterial Agents / biosynthesis*,  metabolism
Bacteriological Techniques / statistics & numerical data
Benzopyrans / analysis,  metabolism
Bioreactors / microbiology
Indoles / analysis,  metabolism
Industrial Microbiology / statistics & numerical data*
Xenorhabdus / metabolism*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Benzopyrans; 0/Indoles; 0/nematophin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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