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Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze.
MedLine Citation:
PMID:  21969788     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To optimize the extraction technology for polysaccharides from the root of Limonium sinense (Girard) Kuntze, Plumbaginaceae and evaluate the antioxidant capacity of polysaccharides from L. sinense (LSEP) MATERIALS AND METHODS: One-singer factor and response surface methodology(RSM) were established to extract the polysaccharides from L. sinense. Then, the 1,1-diphenyl-2-picrylhydrazyl free radical, hydroxyl radical(.OH), and 2,2'-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt free radical assays were established to measure the antioxidant capacity of the LSEP in vitro.
RESULTS: According to analysis, extraction temperature significantly affected extraction yield. The optimum extraction conditions for LSEP were as follows: extraction temperature, 95°C; ultrasonic time 50 minutes; and dosage liquor ratio, 1: 12. Under these conditions, the experimental yield of crude LSEP was 12.80±0.19% which was well matched with the predicted models. The antioxidant capacity data suggested that LSEP has strong antioxidant activity.
CONCLUSION: One-singer factor and RSM were used to extract of LSEP are simple and feasible and LSEP could be developed as a nutraceutical agent for itsstrong antioxidant activity.
Authors:
Xinhui Tang; Lifang Yan; Jing Gao; Haiyan Ge; Haidong Yang; Na Lin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pharmacognosy magazine     Volume:  7     ISSN:  0976-4062     ISO Abbreviation:  Pharmacogn Mag     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-10-04     Completed Date:  2012-10-02     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101300403     Medline TA:  Pharmacogn Mag     Country:  India    
Other Details:
Languages:  eng     Pagination:  186-92     Citation Subset:  -    
Affiliation:
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng - 224002.
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