Document Detail

Optimization of extracellular fungal peroxidase production by 2 Coprinus species.
MedLine Citation:
PMID:  15714234     Owner:  NLM     Status:  MEDLINE    
Optimum culture conditions for the batch production of extracellular peroxidase by Coprinus cinereus UAMH 4103 and Coprinus sp. UAMH 10067 were explored using 2 statistical experimental designs, including 2-level, 7-factor fractional factorial design and 2-factor central composite design. Of the 7 factors examined in the screening study, the concentrations of carbon (glucose) and nitrogen (peptone or casitone) sources showed significant effects on the peroxidase production by Coprinus sp. UAMH 10067. The optimum glucose and peptone concentrations were determined as 2.7% and 0.8% for Coprinus sp. UAMH 10067, and 2.9% and 1.4% for C. cinereus UAMH 4103, respectively. Under the optimized culture condition the maximum peroxidase activity achieved in this study was 34.5 U x mL(-1) for Coprinus sp. UAMH 10067 and 68.0 U x mL(-1) for C. cinereus UAMH 4103, more than 2-fold higher than the results of previous studies.
Keisuke Ikehata; Michael A Pickard; Ian D Buchanan; Daniel W Smith
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  50     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-02-16     Completed Date:  2005-06-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  1033-40     Citation Subset:  IM    
Department of Civil and Environmental Engineering, 3-133 Markin/CNRL Natural Resources Engineering Facility, University of Alberta, Edmonton, Canada.
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MeSH Terms
Carbon / metabolism
Caseins / metabolism
Coprinus / enzymology*,  growth & development,  metabolism
Culture Media / chemistry
Fungal Proteins / biosynthesis,  metabolism
Glucose / metabolism
Nitrogen / metabolism
Peptones / metabolism
Peroxidase / biosynthesis*,  metabolism*
Reg. No./Substance:
0/Caseins; 0/Culture Media; 0/Fungal Proteins; 0/Peptones; 50-99-7/Glucose; 65072-00-6/casein hydrolysate; 7440-44-0/Carbon; 7727-37-9/Nitrogen; EC

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