| Optimization of encoded hydrogel particles for nucleic acid quantification. | |
| | |
MedLine Citation:
|
PMID: 19435332 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The accurate quantification of nucleic acids is of utmost importance for clinical diagnostics, drug discovery, and basic science research. These applications require the concurrent measurement of multiple targets while demanding high-throughput analysis, high sensitivity, specificity between closely related targets, and a wide dynamic range. In attempt to create a technology that can simultaneously meet these demands, we recently developed a method of multiplexed analysis using encoded hydrogel particles. Here, we demonstrate tuning of hydrogel porosity with semi-interpenetrating networks of poly(ethylene glycol), develop a quantitative model to understand hybridization kinetics, and use the findings from these studies to enhance particle design for nucleic acid detection. With an optimized particle design and efficient fluorescent labeling scheme, we demonstrate subattomole sensitivity and single-nucleotide specificity for small RNA targets. |
| | |
Authors:
|
Daniel C Pregibon; Patrick S Doyle |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Analytical chemistry Volume: 81 ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2009 Jun |
Date Detail:
|
Created Date: 2009-06-12 Completed Date: 2009-08-14 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 4873-81 Citation Subset: IM |
Affiliation:
|
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
DNA
/
analysis* Fluorescent Dyes / chemistry Hydrogels / chemistry* Microfluidic Analytical Techniques / instrumentation, methods* Nucleic Acid Hybridization Polyethylene Glycols / chemistry RNA / analysis* |
| Grant Support | |
ID/Acronym/Agency:
|
R21 EB008814-01A1/EB/NIBIB NIH HHS; R21EB008814/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Fluorescent Dyes; 0/Hydrogels; 0/Polyethylene Glycols; 63231-63-0/RNA; 9007-49-2/DNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Convective interaction media monolithic chromatography with ICPMS and ultraperformance liquid chroma...
Next Document: Fourth and fifth virial coefficients of polarizable water.