Document Detail

Optimization of culture conditions for production of cis-epoxysuccinic acid hydrolase using response surface methodology.
MedLine Citation:
PMID:  18083551     Owner:  NLM     Status:  MEDLINE    
Response surface methodology, which allows for rapid identification of important factors and optimization of them to enhance enzyme production, was employed here to optimize culture conditions for the production of cis-epoxysuccinic acid hydrolase from Bordetella sp. strain 1-3. In the first step, a Plackett-Burman design was used to evaluate the effects of nine variables (yeast extract, cis-epoxysuccinic acid, KH(2)PO(4), K(2)HPO(4).3H(2)O, MgSO(4).7H(2)O, trace minerals solution, culture volume, initial pH and incubation time) on the enzyme production. Yeast extract, cis-epoxysuccinic acid and KH(2)PO(4) had significant influences on cis-epoxysuccinic acid hydrolase production and their concentrations were further optimized using central composite design and response surface analysis. A combination of adjusting the concentration of yeast extract to 7.8 g/l, cis-epoxysuccinic acid to 9.8 g/l, and KH(2)PO(4) to 1.12 g/l would favor maximum cis-epoxysuccinic acid hydrolase production. An enhancement of cis-epoxysuccinic acid hydrolase production from 5.6 U/ml to 9.27 U/ml was gained after optimization.
Xia Li; Tongcheng Xu; Xiaohang Ma; Kangping Guo; Lei Kai; Yuhua Zhao; Xiaoming Jia; Yin Ma
Publication Detail:
Type:  Journal Article     Date:  2008-02-20
Journal Detail:
Title:  Bioresource technology     Volume:  99     ISSN:  0960-8524     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-05-12     Completed Date:  2008-08-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  5391-6     Citation Subset:  IM    
College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
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MeSH Terms
Bacterial Proteins / metabolism
Bordetella / enzymology*
Epoxide Hydrolases / metabolism*
Epoxy Compounds / metabolism*
Succinic Acid / metabolism*
Surface Properties
Tartrates / metabolism
Reg. No./Substance:
0/Bacterial Proteins; 0/Epoxy Compounds; 0/Tartrates; 110-15-6/Succinic Acid; 526-83-0/tartaric acid; EC 3.3.2.-/Epoxide Hydrolases

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