Document Detail

Optimization of chitosan treatments for managing microflora in lettuce seeds without affecting germination.
MedLine Citation:
PMID:  23218371     Owner:  NLM     Status:  In-Data-Review    
Many studies have focused on seed decontamination but no one has been capable of eliminating all pathogenic bacteria. Two objectives were followed. First, to assess the in vitro antimicrobial activity of chitosan against: (a) Escherichia coli O157:H7, (b) native microflora of lettuce and (c) native microflora of lettuce seeds. Second, to evaluate the efficiency of chitosan on reducing microflora on lettuce seeds. The overall goal was to find a combination of contact time and chitosan concentration that reduces the microflora of lettuce seeds, without affecting germination. After treatment lettuce seeds presented no detectable microbial counts (<10(2)CFU/50seeds) for all populations. Moreover, chitosan eliminated E. coli. Regardless of the reduction in the microbial load, a 90% reduction on germination makes imbibition with chitosan, uneconomical. Subsequent treatments identified the optimal treatment as 10min contact with a 10g/L chitosan solution, which maintained the highest germination percentage.
M G Goñi; M R Moreira; G E Viacava; S I Roura
Publication Detail:
Type:  Journal Article     Date:  2012-10-06
Journal Detail:
Title:  Carbohydrate polymers     Volume:  92     ISSN:  1879-1344     ISO Abbreviation:  Carbohydr Polym     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8307156     Medline TA:  Carbohydr Polym     Country:  England    
Other Details:
Languages:  eng     Pagination:  817-23     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Concejo Nacional de Ciencia y Tecnología (CONICET), Argentina; Grupo de Investigación en Ingeniería de Alimentos, Facultad de Ingeniería, UNMdP, Juan B. Justo 4302, Mar del Plata 7600, Argentina. Electronic address:
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