| Optimization of α-amylase production by Bacillus subtilis RSKK96: using the Taguchi experimental design approach. | |
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MedLine Citation:
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PMID: 21229466 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, the Taguchi experimental design was applied to optimize the conditions for α-amylase production by Bacillus subtilis RSKK96, which was purchased from Refik Saydam Hifzissihha Industry (RSHM). Four factors, namely, carbon source, nitrogen source, amino acid, and fermentation time, each at four levels, were selected, and an orthogonal array layout of L(16) (4(5)) was performed. The model equation obtained was validated experimentally at maximum casein (1%), corn meal (1%), and glutamic acid (0.01%) concentrations with incubation time to 72 h in the presence of 1% inoculum density. Point prediction of the design showed that maximum α-amylase production of 503.26 U/mg was achieved under optimal experimental conditions. |
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Authors:
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Ersin Uysal; Nurullah Akcan; Zübeyde Baysal; Fikret Uyar |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Preparative biochemistry & biotechnology Volume: 41 ISSN: 1532-2297 ISO Abbreviation: Prep. Biochem. Biotechnol. Publication Date: 2011 |
Date Detail:
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Created Date: 2011-01-13 Completed Date: 2011-06-22 Revised Date: 2012-04-13 |
Medline Journal Info:
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Nlm Unique ID: 9607037 Medline TA: Prep Biochem Biotechnol Country: England |
Other Details:
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Languages: eng Pagination: 84-93 Citation Subset: IM |
Affiliation:
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Dicle University, Diyarbakır Vocational School, Diyarbakır, Turkey. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Bacillus subtilis / enzymology* Caseins Culture Media Fermentation Glutamic Acid Research Design Time Factors Zea mays alpha-Amylases / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Caseins; 0/Culture Media; 56-86-0/Glutamic Acid; EC 3.2.1.1/alpha-Amylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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