Document Detail


Optimization of aeration and agitation rates to improve cellulase-free xylanase production by thermotolerant Streptomyces sp. Ab106 and repeated fed-batch cultivation using agricultural waste.
MedLine Citation:
PMID:  16233409     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Thermostable cellulase-free xylanase was produced by Streptomyces sp. Ab106 using agricultural waste, sugar cane bagasse, as the substrate at 50 degrees C and pH 7.0. The central composite face-centered experimental design was applied to evaluate the optimal agitation and aeration rates in a 5-l fermentor. The highest activity (16.0+/-0.5 IU/ml) was obtained at an aeration rate of 1 vvm and an agitation rate of 150 rpm (k(L)a = 351 h(-1)). Using the repeated fed-batch cultivation technique, the maximum xylanase activity of 32+/-1 IU/ml was obtained during the second cycle of repeated fed-batch culture.
Authors:
Charin Techapun; Naiyatat Poosaran; Masanori Watanabe; Ken Sasaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  95     ISSN:  1389-1723     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2003  
Date Detail:
Created Date:  2005-10-19     Completed Date:  2005-11-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  298-301     Citation Subset:  -    
Affiliation:
Department of Biotechnology, Faculty of Agro-Industry, Chiangmai University, Chiangmai 50100, Thailand.
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