Document Detail

Optimization of effect factors for mycelial growth and exopolysaccharide production by Schizophyllum commune.
MedLine Citation:
PMID:  19156366     Owner:  NLM     Status:  MEDLINE    
This paper is concerned with the optimization of effect factors for mycelial growth and exopolysaccharide production by Schizophyllum commune by one-factor-at-a-time and orthogonal methods. The one-factor-at-a-time method was adopted to investigate the effects of six different compounds (sodium carboxymethylcellulose, L: -glutamic acid, V(B1), naphthalene acetic acid, oleic acid, and Tween 80) on mycelial growth and exopolysaccharide production. Among these factors, oleic acid, V(B1) and Tween 80 were identified to be the most important factors. Subsequently, the concentration of oleic acid, V(B1) and Tween 80 were optimized using the orthogonal matrix method. The effects of the factors on the mycelial growth of S. commune were in the order of oleic acid > V(B1) > Tween 80, and those on exopolysaccharide production were in the same order. The optimal concentration for mycelia and exopolysaccharide were determined as oleic acid 0.1% (v/v), V(B1) 0.5 mg/L, and Tween 80 6 mg/L. The subsequent verification experiments confirmed the validity of the models. Under this optimized conditions in shake flask culture, the mycelial yield and exo-biopolymer production were 25.93 and 2.79 g/L, respectively, which were considerably higher than those obtained in the preliminary studies. The result was further confirmed in a 7-L fermentor experiments.
Li-min Hao; Xin-hui Xing; Zheng Li; Jian-chun Zhang; Jin-xu Sun; Shi-ru Jia; Chang-sheng Qiao; Tianyi Wu
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Publication Detail:
Type:  Journal Article     Date:  2009-01-21
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  160     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-26     Completed Date:  2010-04-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  621-31     Citation Subset:  IM    
Department of Chemical Engineering, Tsinghua University, Beijing, People's Republic of China.
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MeSH Terms
Culture Media / chemistry,  metabolism*
Extracellular Space / metabolism*
Mycelium / growth & development*,  metabolism
Polysaccharides / metabolism*
Schizophyllum / growth & development,  metabolism*
Reg. No./Substance:
0/Culture Media; 0/Polysaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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