Document Detail


Optimization of EMIT reagent system using a COBAS-BIO centrifugal analyzer.
MedLine Citation:
PMID:  7033663     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An optimized method for application of the EMIT reagent system to a COBAS-BIO centrifugal analyzer was developed to minimize reagent consumption while maintaining good precision and accuracy. Forty-one serum samples, previously analyzed for theophylline by HPLC, were assayed using the modified protocol on the COBAS-BIO centrifugal analyzer. Regression analysis of the results obtained from both methods yielded the following equation: y = 1.10x + 0.01, r = 0.964 (x = HPLC, y = COBAS-BIO centrifugal analyzer, EMIT). The COBAS-BIO centrifugal analyzer and EMIT methodology provided an efficient, inexpensive, and relatively effortless approach to drug monitoring in the routine clinical laboratory.
Authors:
C N Ou; V L Frawley; G J Buffone
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of analytical toxicology     Volume:  5     ISSN:  0146-4760     ISO Abbreviation:  J Anal Toxicol     Publication Date:    1981 Sep-Oct
Date Detail:
Created Date:  1982-03-26     Completed Date:  1982-03-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705085     Medline TA:  J Anal Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  249-52     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anticonvulsants / blood
Centrifugation / instrumentation*
Chromatography, High Pressure Liquid
Humans
Immunoenzyme Techniques / instrumentation*
Indicators and Reagents
Pharmaceutical Preparations / blood*
Theophylline / blood
Chemical
Reg. No./Substance:
0/Anticonvulsants; 0/Indicators and Reagents; 0/Pharmaceutical Preparations; 58-55-9/Theophylline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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