Document Detail

Optimization of EMIT reagent system using a COBAS-BIO centrifugal analyzer.
MedLine Citation:
PMID:  7033663     Owner:  NLM     Status:  MEDLINE    
An optimized method for application of the EMIT reagent system to a COBAS-BIO centrifugal analyzer was developed to minimize reagent consumption while maintaining good precision and accuracy. Forty-one serum samples, previously analyzed for theophylline by HPLC, were assayed using the modified protocol on the COBAS-BIO centrifugal analyzer. Regression analysis of the results obtained from both methods yielded the following equation: y = 1.10x + 0.01, r = 0.964 (x = HPLC, y = COBAS-BIO centrifugal analyzer, EMIT). The COBAS-BIO centrifugal analyzer and EMIT methodology provided an efficient, inexpensive, and relatively effortless approach to drug monitoring in the routine clinical laboratory.
C N Ou; V L Frawley; G J Buffone
Related Documents :
25442573 - Development of pressurised hot water extraction (phwe) for essential compounds from mor...
19911173 - Immunoassay detection in a perfluorocarbon emulsion oxygen therapeutic.
20945833 - High-performance isotope labeling for profiling carboxylic acid-containing metabolites ...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of analytical toxicology     Volume:  5     ISSN:  0146-4760     ISO Abbreviation:  J Anal Toxicol     Publication Date:    1981 Sep-Oct
Date Detail:
Created Date:  1982-03-26     Completed Date:  1982-03-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705085     Medline TA:  J Anal Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  249-52     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anticonvulsants / blood
Centrifugation / instrumentation*
Chromatography, High Pressure Liquid
Immunoenzyme Techniques / instrumentation*
Indicators and Reagents
Pharmaceutical Preparations / blood*
Theophylline / blood
Reg. No./Substance:
0/Anticonvulsants; 0/Indicators and Reagents; 0/Pharmaceutical Preparations; 58-55-9/Theophylline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  An update of approaches toward the fluorescence immunoassay of drugs.
Next Document:  Chuchuhuasha - a drug used in folk medicine in the Amazonian and Andean areas. A chemical study of M...