| Optimization of Cold-Active Lipase Production from Psychrophilic Bacterium Moritella sp. 2-5-10-1 by Statistical Experimental Methods. | |
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MedLine Citation:
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PMID: 23291744 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Statistical experimental designs were applied to optimize cold-active lipase production by the psychrophilic bacterium Moritella sp. 2-5-10-1. First, a Plackett-Burmen design (PBD) was used to evaluate the significant effects of various fermentation parameters. The results indicated that soybean meal, temperature, and Tween-80 had significant influences on lipase production. The levels of these variables were optimized subsequently using central composite design (CCD). A quadratic regression model of cold-active lipase production was built, and verification experiments confirmed its validity. On subsequent scale-up in a 10-L bioreactor using optimized conditions, cold-active lipase production (30.56 U/mL) was obtained. The results clearly indicated that the model was adequate even on a large scale. To our knowledge, this is the first report of statistical optimization of cold-active lipase production by a psychrophilic bacterium. |
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Authors:
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Quanfu Wang; Chunyu Zhang; Yanhua Hou; Xuezheng Lin; Jihong Shen; Xiangyu Guan |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: - ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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School of Marine and Technology, Harbin Institute of Technology. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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