| Optimization of Cell Lysis and Protein Digestion Protocols for the Analysis of HeLa S3 Cells by LC-MS/MS. | |
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MedLine Citation:
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PMID: 22002805 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In order to maximize the number of proteins identified from Hela S3 cell lysate we tested various cell lysis, protein precipitation and digestion protocols. First, we compared 3 different lysis buffers, 2 mechanical cell disruption methods and 2 precipitation methods. Then we tested 6 different in-solution digestion protocols, 3 different in-gel digestion protocols and 10 different peptide extraction protocols. The result is a proposal for an optimized protocol to prepare whole cell lysate samples from HeLa S3 cells. |
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Authors:
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Dominic Winter; Hanno Steen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-17 |
Journal Detail:
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Title: Proteomics Volume: - ISSN: 1615-9861 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Departments of Pathology, Children's Hospital Boston and Harvard Medical School, Boston, MA, USA; Proteomics Center at Children's Hospital Boston, Boston, MA, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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