Document Detail

Optimization of Brewery's spent grain dilute-acid hydrolysis for the production of pentose-rich culture media.
MedLine Citation:
PMID:  15054253     Owner:  NLM     Status:  MEDLINE    
Dilute-acid hydrolysis of brewery's spent grain to obtain a pentose-rich fermentable hydrolysate was investigated. The influence of operational conditions on polysaccharide hydrolysis was assessed by the combined severity parameter (CS) in the range of 1.39-3.06. When the CS increased, the pentose sugars concentration increased to a maximum at a CS of 1.94, whereas the maximum glucose concentration was obtained for a CS of 2.65. The concentrations of furfural, hydroxymethylfurfural (HMF), as well as formic and levulinic acids and total phenolic compounds increased with severity. Optimum hydrolysis conditions were found at a CS of 1.94 with >95% of feedstock pentose sugars recovered in the monomeric form, together with a low content of furfural, HMF, acetic and formic acids, and total phenolic compounds. This hydrolysate containing glucose, xylose, and arabinose (ratio 10:67:32) was further supplemented with inorganic salts and vitamins and readily fermented by the yeast Debaryomyces hansenii CCMI 941 without any previous detoxification stage. The yeast was able to consume all sugars, furfural, HMF, and acetic acid with high biomass yield, 0.68 C-mol/C-mol, and productivity, 0.92 g/(L.h). Detoxification with activated charcoal resulted in a similar biomass yield and a slight increase in the volumetric productivity (11%).
Florbela Carvalheiro; Luís C Duarte; Raquel Medeiros; Francisco M Gírio
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  113-116     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2004  
Date Detail:
Created Date:  2004-03-31     Completed Date:  2004-07-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1059-72     Citation Subset:  IM    
INETI, Departamento de Biotecnologia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal.
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MeSH Terms
Alcoholic Beverages
Amino Acids / chemistry
Ascomycota / metabolism
Biotechnology / methods*
Cell Division
Cereals / chemistry*
Chromatography, Ion Exchange
Culture Media / chemistry*
Hydrogen-Ion Concentration
Industrial Waste
Ion Exchange Resins
Models, Statistical
Pentoses / chemistry*
Phenol / chemistry
Polysaccharides / chemistry
Time Factors
Xylitol / chemistry
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Culture Media; 0/Industrial Waste; 0/Ion Exchange Resins; 0/Pentoses; 0/Polysaccharides; 0/casamino acids; 108-95-2/Phenol; 8024-50-8/hemicellulose; 87-99-0/Xylitol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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