| Optimization of Brewery's spent grain dilute-acid hydrolysis for the production of pentose-rich culture media. | |
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MedLine Citation:
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PMID: 15054253 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dilute-acid hydrolysis of brewery's spent grain to obtain a pentose-rich fermentable hydrolysate was investigated. The influence of operational conditions on polysaccharide hydrolysis was assessed by the combined severity parameter (CS) in the range of 1.39-3.06. When the CS increased, the pentose sugars concentration increased to a maximum at a CS of 1.94, whereas the maximum glucose concentration was obtained for a CS of 2.65. The concentrations of furfural, hydroxymethylfurfural (HMF), as well as formic and levulinic acids and total phenolic compounds increased with severity. Optimum hydrolysis conditions were found at a CS of 1.94 with >95% of feedstock pentose sugars recovered in the monomeric form, together with a low content of furfural, HMF, acetic and formic acids, and total phenolic compounds. This hydrolysate containing glucose, xylose, and arabinose (ratio 10:67:32) was further supplemented with inorganic salts and vitamins and readily fermented by the yeast Debaryomyces hansenii CCMI 941 without any previous detoxification stage. The yeast was able to consume all sugars, furfural, HMF, and acetic acid with high biomass yield, 0.68 C-mol/C-mol, and productivity, 0.92 g/(L.h). Detoxification with activated charcoal resulted in a similar biomass yield and a slight increase in the volumetric productivity (11%). |
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Authors:
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Florbela Carvalheiro; Luís C Duarte; Raquel Medeiros; Francisco M Gírio |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 113-116 ISSN: 0273-2289 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2004 |
Date Detail:
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Created Date: 2004-03-31 Completed Date: 2004-07-26 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 1059-72 Citation Subset: IM |
Affiliation:
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INETI, Departamento de Biotecnologia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alcoholic Beverages Amino Acids / chemistry Ascomycota / metabolism Biotechnology / methods* Carbohydrates Cell Division Cereals / chemistry* Chromatography, Ion Exchange Culture Media / chemistry* Fermentation Hydrogen-Ion Concentration Hydrolysis* Industrial Waste Ion Exchange Resins Kinetics Models, Statistical Pentoses / chemistry* Phenol / chemistry Polysaccharides / chemistry Temperature Time Factors Xylitol / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbohydrates; 0/Culture Media; 0/Industrial Waste; 0/Ion Exchange Resins; 0/Pentoses; 0/Polysaccharides; 0/casamino acids; 108-95-2/Phenol; 8024-50-8/hemicellulose; 87-99-0/Xylitol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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