Document Detail

Optimization of biodiesel production catalyzed by fungus cells immobilized in fibrous supports.
MedLine Citation:
PMID:  19777379     Owner:  NLM     Status:  MEDLINE    
A circulating packed-bed bioreactor system using fibrous nonwoven fabric as the immobilization matrix was suitable for simultaneous cell growth and immobilization of Rhizopus oryzae fungus cells, which could be used for lipase-mediated production of biodiesel by methanolysis of soybean oil. Response surface methodology and 5-level-5-factor central composite rotatable design was proved to be a powerful tool for the optimization of methanolysis conditions catalyzed by immobilized R. oryzae whole cell biocatalyst. A quadratic polynomial regression model was used to analyze the relationship between the yield and the significant reaction parameters. The analysis confirmed that water content, molar ratio of methanol to oil, cell weight, and reaction time were the significant factors affecting the yield at a 95% confidence level (p < 0.05). Under the optimum condition at 10.97% (w/w) water content, 0.64 molar ratio of methanol to oil, 2.25% (w/w) cell weight, and 23.3 h reaction time, the predicted value of yield was 72.6%. Validation experiments with yields of 70.77 +/- 2.46% verified the availability and the accuracy of the model.
Jyh-Ping Chen; Gen-Hsu Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-24
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  161     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-11     Completed Date:  2010-05-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  181-94     Citation Subset:  IM    
Department of Chemical and Materials Engineering, Chang Gung University, Kwei-San, Taoyuan 333, Taiwan, Republic of China.
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MeSH Terms
Cell Culture Techniques
Fungal Proteins / metabolism*
Lipase / metabolism*
Models, Theoretical
Rhizopus / metabolism*
Soybean Oil / metabolism
Reg. No./Substance:
0/Biofuels; 0/Fungal Proteins; 8001-22-7/Soybean Oil; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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