| Optimisation of poly-beta-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems. | |
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MedLine Citation:
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PMID: 15861796 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Poly-beta-hydroxyalkanoate (PHA) is a polymer commonly used in carbon and energy storage for many different bacterial cells. Polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs), store PHA anaerobically through metabolism of carbon substrates such as acetate and propionate. Although poly-beta-hydroxybutyrate (PHB) and poly-beta-hydroxyvalerate (PHV) are commonly quantified using a previously developed gas chromatography (GC) method, poly-beta-hydroxy-2-methylvalerate (PH2MV) is seldom quantified despite the fact that it has been shown to be a key PHA fraction produced when PAOs or GAOs metabolise propionate. This paper presents two GC-based methods modified for extraction and quantification of PHB, PHV and PH2MV from enhanced biological phosphorus removal (EBPR) systems. For the extraction of PHB and PHV from acetate fed PAO and GAO cultures, a 3% sulfuric acid concentration and a 2-20 h digestion time is recommended, while a 10% sulfuric acid solution digested for 20h is recommended for PHV and PH2MV analysis from propionate fed EBPR systems. |
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Authors:
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Adrian Oehmen; Beatrice Keller-Lehmann; Raymond J Zeng; Zhiguo Yuan; Jürg Keller |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1070 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-05-02 Completed Date: 2005-09-29 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 131-6 Citation Subset: IM |
Affiliation:
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Advanced Wastewater Management Centre, The University of Queensland, St. Lucia, Brisbane 4072, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkanes
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analysis* Gas Chromatography-Mass Spectrometry / methods* Phosphorus / isolation & purification* Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
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0/Alkanes; 7723-14-0/Phosphorus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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