| Optimisation of an enteric coated, layered multi-particulate formulation for ileal delivery of viable recombinant Lactococcus lactis. | |
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MedLine Citation:
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PMID: 18423993 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Layering of pellets with recombinant Lactococcus lactis Thy 12 was optimised for the production of a dosage form with a high load of viable recombinant L. lactis. Shear stress induced during the atomisation and the type of carrier used for the layering process did not influence the viability. A 5% lactose matrix resulted in the highest viability of L. lactis (8.9+/-1.7%) which could be maintained for at least 12 months at -20 degrees C. A higher bacterial cell load on the pellets was obtained using a longer process time, but the addition of 10% skim milk was essential to maintain the stabilising capacity of the matrix. Increasing the load of viable L. lactis was also possible using a higher bacterial cell concentration of the layering suspension and increasing the amount of stabilising matrix to 10% lactose/20% skim milk, yielding a formulation with 1.7 x 10(9)cfu/100 mg pellets. To protect the bacteria during gastric passage and to obtain ileum targeting, the formulation was enteric coated with 5% Eudragit FS30D, but after coating and gastric residence for 2 h HCl about 1% of the bacteria remained viable. Application of a subcoating, previous to enteric coating, did not result in a higher viability. |
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Authors:
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Nele Poelvoorde; Nathalie Huyghebaert; Chris Vervaet; Jean-Paul Remon |
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Publication Detail:
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Type: Journal Article Date: 2008-02-29 |
Journal Detail:
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Title: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V Volume: 69 ISSN: 0939-6411 ISO Abbreviation: Eur J Pharm Biopharm Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-04 Completed Date: 2008-10-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9109778 Medline TA: Eur J Pharm Biopharm Country: Netherlands |
Other Details:
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Languages: eng Pagination: 969-76 Citation Subset: IM |
Affiliation:
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Laboratory of Pharmaceutical Technology, Ghent University, Ghent, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cellulose Chemistry, Pharmaceutical Crohn Disease / therapy Culture Media Drug Delivery Systems Excipients Humans Ileum / metabolism* Interleukin-10 / genetics, metabolism Lactococcus lactis / chemistry* Milk / chemistry Polymethacrylic Acids Polyvinyl Alcohol / chemistry Starch Stomach / metabolism Tablets, Enteric-Coated Thymidine / administration & dosage |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Excipients; 0/Polymethacrylic Acids; 0/Tablets, Enteric-Coated; 0/microcrystalline cellulose; 130068-27-8/Interleukin-10; 25086-15-1/methylmethacrylate-methacrylic acid copolymer; 50-89-5/Thymidine; 9002-89-5/Polyvinyl Alcohol; 9004-34-6/Cellulose; 9005-25-8/Starch |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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