Document Detail

Optimisation of the cryopreservation of primary hepatocytes.
MedLine Citation:
PMID:  20645047     Owner:  NLM     Status:  MEDLINE    
The use of cryopreserved hepatocytes has increased in the last decade due to the improvement of the freezing and thawing methods and has even achieved acceptance by the U.S. Food and Drug Administration for use in drug-metabolising enzyme induction studies. This chapter provides an overview of the theories behind the process of cryopreservation and some of the most important advances which have led to the ability to cryopreserve hepatocytes, which when thawed retain functions similar to fresh cells. Parameters such as cell density, cryoprotectants and freezing media should be considered as well as storage conditions and thawing techniques. Special emphasis is placed on human hepatocytes but information for the cryopreservation of animal hepatocytes is also described. Finally, a suggested method for optimising cryopreservation method is outlined.
Nicola J Hewitt
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  640     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-20     Completed Date:  2010-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  83-105     Citation Subset:  IM    
Scientific Writing Services, Erzhausen, Germany.
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MeSH Terms
Cells, Cultured
Cryopreservation / methods*
Cryoprotective Agents
Hepatocytes / cytology*
Reg. No./Substance:
0/Cryoprotective Agents

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