| Optimisation of ambient and high temperature asymmetric flow field-flow fractionation with dual/multi-angle light scattering and infrared/refractive index detection. | |
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MedLine Citation:
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PMID: 20022331 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Asymmetric flow field-flow fractionation (AF4) enables to analyse polymers with very high molar masses under mild conditions in comparison to size exclusion chromatography (SEC). Conventionally, membranes for AF4 are made from cellulose. Recently, a novel ceramic membrane has been developed which can withstand high temperatures above 130 degrees C and chlorinated organic solvents, thus making it possible to characterise semicrystalline polyolefins by HT-AF4. Two ceramic membranes and one cellulose membrane were compared with regard to their quality of molar mass separation and the loss of the polymer material through the pores. Separating polystyrene standards as model compounds at different cross-flow gradients the complex relationship between cross-flow velocity, separation efficiency, the molar mass and peak broadening could be elucidated in detail. Moreover, the dependence of signal quality and reproducibility on sample concentration and mass loading was investigated because the evaluation of the obtained fractograms substantially depends on the signal intensities. Finally, the performance of the whole system was tested at high temperature by separating PE reference materials of high molar mass. |
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Authors:
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T Otte; R Br?ll; T Macko; H Pasch; T Klein |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-06 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1217 ISSN: 1873-3778 ISO Abbreviation: J Chromatogr A Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-28 Completed Date: 2010-05-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 722-30 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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German Institute for Polymers, Schlossgartenstrasse 6, 64289 Darmstadt, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ceramics
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chemistry Chromatography, Gel Fractionation, Field Flow / methods* Light Polyenes / chemistry Refractometry Scattering, Radiation* Shear Strength Spectrophotometry, Infrared / methods* Temperature |
| Chemical | |
Reg. No./Substance:
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0/Ceramics; 0/Polyenes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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