Document Detail


Optimal use of photomultipliers for chemiluminescence and bioluminescence applications.
MedLine Citation:
PMID:  10398555     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photomultipliers are the preferred detectors for quantifying weak or short-lived light emissions. The quality of signal recovery has an upper limit imposed by the statistical nature of light detection. It is shown that the photon counting method can provide performance close to ideal, whereas performance in the current measuring method is degraded by noise in the multiplier gain process and by the nature of the dark current. Performance variations because of changes in temperature, shock, vibration and magnetic field effects are significantly reduced when using the photon counting method. The effects of dead-time can be corrected making photon counting superior to current measuring with regard to dynamic range. The benefits of using photon counting packages are presented.
Authors:
R J Ellis; A G Wright
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Luminescence : the journal of biological and chemical luminescence     Volume:  14     ISSN:  1522-7235     ISO Abbreviation:  Luminescence     Publication Date:    1999 Jan-Feb
Date Detail:
Created Date:  2000-06-08     Completed Date:  2000-06-08     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  100889025     Medline TA:  Luminescence     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  11-8     Citation Subset:  IM    
Copyright Information:
Copyright 1999 John Wiley & Sons, Ltd.
Affiliation:
Electron Tubes Ltd, Bury Street, Ruislip HA4 7TA, UK.
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MeSH Terms
Descriptor/Qualifier:
Chemiluminescent Measurements*
Light
Luminescence*
Photometry / methods*
Photons

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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