Document Detail


Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes.
MedLine Citation:
PMID:  21645890     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE: To develop and test a simple, feasible approach to improve spindle assembly and developmental competence of human in vitro matured oocytes by parthenogenetic activation in maturation medium. DESIGN: Prospective, randomized study. SETTING: University research laboratory-based assisted reproductive technology laboratory. PATIENT(S): Four hundred thirty-two patients with male factor infertility undergoing intracytoplasmic sperm injection (ICSI) cycles. INTERVENTION(S): In vivo failed-to-mature oocytes from the ICSI cycles were divided into four groups according to differing duration after the extrusion of first polar body (0-1, 2-3, 4-5, and 8-9 hours). Oocytes spindles in each group were immunostained for α-tubulin and chromosomes, and observed by confocal microscopy. MAIN OUTCOME MEASURE(S): Rate from pronuclear stage to blastocyst, embryo grading at the eight-cell and blastocyst stages, and spindle assembly. RESULT(S): There was a statistically significantly higher rate of development at the eight-cell and blastocyst stages in the 2- to 3-hour and 4- to 5-hour groups. The grading results at the eight-cell and blastocyst stages also showed that the proportion of embryos of high quality was similar among the 2- to 3-hour and 4- to 5-hour groups, and in these groups it was statistically significantly higher than the 0- to 1-hour and 8- to 9-hour groups. The results of immunofluorescence demonstrated that there was a statistically significantly higher rate of normal spindle assembly in the 2- to 3-hour and 4- to 5-hour groups. CONCLUSION(S): Optimal timing of maturation benefits the development of competence of in vitro matured oocytes by promoting normal spindle assembly.
Authors:
Yang Yu; Jie Yan; Zi C Liu; Li Y Yan; Min Li; Qi Zhou; Jie Qiao
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-4
Journal Detail:
Title:  Fertility and sterility     Volume:  -     ISSN:  1556-5653     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Beijing, People's Republic of China; Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, People's Republic of China; Graduate School, Chinese Academy of Sciences, Beijing, People's Republic of China.
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