| Optimal selection of 2D reversed-phase-reversed-phase HPLC separation techniques in bottom-up proteomics. | |
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MedLine Citation:
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PMID: 22462784 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Evaluation of: Stephanowitz H, Lange S, Lang D et al. Improved two-dimensional reversed phase-reversed phase LC-MS/MS approach for identification of peptide-protein interactions. J. Proteome Res. 11(2), 1175-1183 (2011). Recent developments in bottom-up proteomics have supplanted the use of gel-based approaches in favor of multidimensional chromatographic separations of peptide mixtures followed by mass spectrometry analysis. This trend is driven by the desire to eliminate labor-intensive in-gel digestion procedures and increase proteome coverage through better recovery of proteolytic fragments. Introduction of reversed-phase-reversed-phase 2D separation techniques is one of the major improvements that have made this possible. In this article, we review recent developments in 2D HPLC and highlight variations in reversed-phase HPLC separation selectivity that allow for superior peak capacity in peptide fractionation. |
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Authors:
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Peter McQueen; Oleg Krokhin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Expert review of proteomics Volume: 9 ISSN: 1744-8387 ISO Abbreviation: Expert Rev Proteomics Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-04-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101223548 Medline TA: Expert Rev Proteomics Country: England |
Other Details:
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Languages: eng Pagination: 125-8 Citation Subset: IM |
Affiliation:
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Manitoba Centre for Proteomics & Systems Biology, University of Manitoba, 799 JBRC, 715 McDermot Avenue, Winnipeg, MB, R3E 3P4, Canada. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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