| Optimal production and in vitro activity of recombinant endostatin from stably transformed Drosophila melanogaster S2 cells. | |
| | |
MedLine Citation:
|
PMID: 11249205 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Recombinant plasmids containing a complementary deoxyribonucleic acid coding mouse endostatin were transfected and stably expressed in Drosophila melanogaster Schneider 2 (S2) cells. Stably transformed polyclonal cell populations expressing recombinant endostatin were isolated after 4 wk of selection with hygromycin B. Recombinant endostatin expressed in the stably transformed S2 cells under the influence of the Drosophila BiP protein signal sequence was secreted into the medium. Recombinant endostatin was also purified to homogeneity using a simple one-step Ni2+ affinity fractionation method. Purified recombinant endostatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at maximum inhibition for recombinant endostatin was approximately 1.8 microg/ml. The stably transformed S2 cells produced 18 mg recombinant endostatin/L 7 d after induction with 5 microM CdCl2. Sodium butyrate supplementation (2.5 mM) increased recombinant endostatin production by 17%. These findings demonstrate optimal production and in vitro activity of recombinant endostatin from stably transformed D. melanogaster S2 cells. |
| | |
Authors:
|
J H Park; K H Chang; J M Lee; Y H Lee; I S Chung |
Related Documents
:
|
2835515 - Isolation of a transformation-defective mutant of the mcdonough strain of feline sarcom... 8557695 - Selective activation of t cell kinase p56lck by herpesvirus saimiri protein tip. 3670325 - Differential sensitivities of transformed and untransformed murine cell lines to dna cr... 2434835 - Detection of phosphotyrosine-containing proteins in polyomavirus middle tumor antigen-t... 16346715 - Bacterial o-methylation of chloroguaiacols: effect of substrate concentration, cell den... 23292035 - Improved lithium-sulfur cells with a treated carbon paper interlayer. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: In vitro cellular & developmental biology. Animal Volume: 37 ISSN: 1071-2690 ISO Abbreviation: In Vitro Cell. Dev. Biol. Anim. Publication Date: 2001 Jan |
Date Detail:
|
Created Date: 2001-03-15 Completed Date: 2001-06-07 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: United States |
Other Details:
|
Languages: eng Pagination: 5-9 Citation Subset: IM |
Affiliation:
|
Department of Genetic Engineering, Kyung Hee University, Suwon, South Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Angiogenesis Inhibitors
/
biosynthesis*,
genetics Animals Blotting, Western Butyrates / pharmacology Cadmium / pharmacology Cells, Cultured Collagen / biosynthesis*, genetics DNA, Complementary / isolation & purification Drosophila melanogaster / genetics*, metabolism Electrophoresis, Polyacrylamide Gel Endostatins Metallothionein / genetics Mice Peptide Fragments / biosynthesis*, genetics Promoter Regions, Genetic Recombinant Proteins / biosynthesis, genetics |
| Chemical | |
Reg. No./Substance:
|
0/Angiogenesis Inhibitors; 0/Butyrates; 0/DNA, Complementary; 0/Endostatins; 0/Peptide Fragments; 0/Recombinant Proteins; 7440-43-9/Cadmium; 9007-34-5/Collagen; 9038-94-2/Metallothionein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulation of retinal capillary cells by basic fibroblast growth factor, vascular endothelial growth...
Next Document: Pleiotropic effects of corticotropin releasing hormone on normal human skin keratinocytes.