Document Detail


Optimal perfusion of an intact ovary as a prerequisite for successful ovarian cryopreservation.
MedLine Citation:
PMID:  18056117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Cryopreservation and subsequent reimplantation of intact ovaries from cancer patients, offers potentially the best prognosis for restoring fertility after sterilizing cancer treatment. We used bovine ovaries as a model system to explore the perfusion procedure that is required for cryopreservation of intact ovaries. METHODS: The arteria ovarica was cannuled, and ovaries were flushed with Indian ink for 5 min. RESULTS: Successful perfusion of blood vessels was immediately visible macroscopically by a grey to black discoloration of the ovary and was confirmed microscopically, by examining tissue sections. There was no correlation between the time interval from removal of the ovary to the start of the perfusion, and success of perfusion. We determined the percentage of Indian ink-perfused vessels and scored blood vessels in four different size classes. The percentage of perfused vessels increased with an increase in vessel size. In a limited set of preliminary experiments with human ovaries, comparable results were obtained. CONCLUSIONS: Our results show that bovine ovaries are a suitable and adequate model system for optimizing the cryopreservation of human ovaries. As bovine are at least of comparable size to human ovaries, we expect that our results can be extrapolated to the human situation.
Authors:
R Gerritse; C C M Beerendonk; M S L Tijink; A Heetkamp; J A M Kremer; D D M Braat; J R Westphal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-02
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  23     ISSN:  1460-2350     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-17     Completed Date:  2008-03-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  329-35     Citation Subset:  IM    
Affiliation:
Streekziekenhuis Koningin Beatrix, Department of Obstetrics and Gynecology, Beatrixpark 1, 7101 BN Winterswijk, The Netherlands. r.gerritse@skbwinterswijk.nl
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Carbon
Cattle
Cryopreservation*
Female
Humans
Immunohistochemistry / methods
Models, Animal
Ovary / blood supply*
Perfusion*
Staining and Labeling
Swine
Chemical
Reg. No./Substance:
0/chinese ink; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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