Document Detail


Optimal detection of oligoclonal bands in CSF by silver stain.
MedLine Citation:
PMID:  6195553     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We applied a sensitive silver nitrate stain to SDS-polyacrylamide gels to detect oligoclonal bands in unconcentrated CSF. Silver staining took less than 3 hours and gave easily readable bands in 93% of MS patients. In contrast, standard Coomassie Blue stain was positive in only 53%. Maximum resolution and numbers of bands resulted when silver staining was used on 10 to 20 microliters of CSF. Silver staining of SDS-polyacrylamide gels permits reliable oligoclonal band detection on small volumes of unconcentrated CSF.
Authors:
P K Coyle; C Johnson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurology     Volume:  33     ISSN:  0028-3878     ISO Abbreviation:  Neurology     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1983-12-17     Completed Date:  1983-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401060     Medline TA:  Neurology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1510-2     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Electrophoresis, Polyacrylamide Gel*
Humans
Immunoglobulin G / cerebrospinal fluid
Immunoglobulins / cerebrospinal fluid*
Multiple Sclerosis / cerebrospinal fluid*
Silver Nitrate / diagnostic use
Staining and Labeling
Chemical
Reg. No./Substance:
0/Immunoglobulin G; 0/Immunoglobulins; 7761-88-8/Silver Nitrate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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