Document Detail

Optimal conditions for cryopreservation of primary chicken embryo kidney cells with dimethyl sulfoxide.
MedLine Citation:
PMID:  17652787     Owner:  NLM     Status:  MEDLINE    
Conditions were evaluated for optimum cryopreservation of primary chicken embryo kidney (CEK) cells. The recovery of viable CEK cells was best (50.8% viability) when the concentration of dimethyl sulfoxide (DMSO) in the freezing medium was 20% (v/v). The viability of primary CEK cells was not influenced by the concentration of calf serum in the freezing medium, the duration of storage at -70 degrees C before storage in liquid nitrogen, cell concentration, or the method of addition or dilution of DMSO. Thawed cells recovered and grew in complete growth medium similarly to cells freshly isolated from kidney, and influenza viruses produced plaques in the monolayer. The cryopreservation procedures described here may facilitate maintenance of a standard stock of primary CEK cells for laboratories where preparation of primary CEK cells is not an option.
Jee-Wan Choi; Eun-Kyung Shin; Suk-Hoon Ha; Hyun Ah Kim; Yeon Hee Kim; Jae Seoung Kim; Tae-Wook Hahn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular biotechnology     Volume:  35     ISSN:  1073-6085     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-07-26     Completed Date:  2007-09-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  237-41     Citation Subset:  IM    
School of Veterinary Medicine, Kangwon National University, 192-1 Hyoja-dong Chuncheon, Kangwon-Do, 200-701, South Korea.
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MeSH Terms
Cells, Cultured
Chick Embryo
Dimethyl Sulfoxide / pharmacology*
Kidney / cytology,  embryology*
Reg. No./Substance:
67-68-5/Dimethyl Sulfoxide

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