Document Detail


Optimal aggregation of dissociated islet cells for functional islet-like cluster.
MedLine Citation:
PMID:  18318957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genetically-engineered islets can serve as a potential means to modulate the regional immunity occurring in the transplant environment, although gene transfer into islets is not easy due to the extracellular matrix of islets. In this study, we aggregated single islet cells dissociated by trypsinization for successful gene transfection into islet cells. To enhance the functionality of aggregated islets, we optimized the conditions for the aggregation of dissociated islet cells using several supplements such as collagen, all-trans-retinoic acid (atRA) and the culture medium of pancreatic exocrine cells (ECM). The dissociated islet cells formed tight cell clusters containing the normal insulin secretion against glucose concentration and the aggregation yield was significantly improved by treatment with atRA and ECM. However, collagen did not improve cell aggregation. Therefore, this re-aggregation technology would be useful for the development of genetically-engineered islets.
Authors:
Dong Yun Lee; Sang Jin Park; Jong Hee Nam; Youngro Byun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomaterials science. Polymer edition     Volume:  19     ISSN:  0920-5063     ISO Abbreviation:  J Biomater Sci Polym Ed     Publication Date:  2008  
Date Detail:
Created Date:  2008-03-05     Completed Date:  2008-06-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9007393     Medline TA:  J Biomater Sci Polym Ed     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  441-52     Citation Subset:  IM    
Affiliation:
College of Pharmacy, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul 151-742, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Cell Aggregation
Collagen / metabolism
Culture Media
Insulin / secretion
Islets of Langerhans / cytology*,  secretion
Pancreas, Exocrine / cytology,  metabolism
Tretinoin / metabolism
Chemical
Reg. No./Substance:
0/Culture Media; 11061-68-0/Insulin; 302-79-4/Tretinoin; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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