Document Detail

Optimal access to the rat heart by transverse bilateral thoracotomy with double ligature of the internal thoracic arteries.
MedLine Citation:
PMID:  18947170     Owner:  NLM     Status:  MEDLINE    
Rats are used widely in ischemia-reperfusion and other heart experiments, but current protocols for thoracotomy have serious shortcomings. Median sternotomy causes bleeding from sternum itself and the internal thoracic arteries, whereas left thoracotomy requires exteriorization of the heart and its reintroduction after completion of the procedure and often is complicated by traction or torsion of the cardiopulmonary bundle and atelectasis in the left lung. Here we describe a new, terminal procedure that minimizes blood loss and allows wide access to the heart without disturbing its anatomic position. Transverse thoracotomy, preferably through the fifth intercostal space, is performed after double ligature of both internal thoracic arteries 1 intercostal space above and 1 below the incision. Blood loss is minimal and occurs mainly with dissection of deep pectoral muscles and intercostal muscles, and the animal is better ventilated than with conventional protocols. We believe that our procedure is superior to existing techniques because it minimizes blood loss during intervention, does not disturb the anatomic position of the heart, and allows wide access to the organ for experimental manipulation.
Valentin L Ordodi; Virgil Paunescu; Felix A Mic
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of the American Association for Laboratory Animal Science : JAALAS     Volume:  47     ISSN:  1559-6109     ISO Abbreviation:  J. Am. Assoc. Lab. Anim. Sci.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-10-24     Completed Date:  2008-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  101269489     Medline TA:  J Am Assoc Lab Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  44-6     Citation Subset:  IM    
Department of Physiology, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
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MeSH Terms
Cardiac Surgical Procedures / methods*
Rats, Sprague-Dawley
Thoracic Arteries*
Thoracotomy / methods*

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