Document Detail


Optically micropatterned culture of adherent cells.
MedLine Citation:
PMID:  22894476     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
We used a liquid-crystal spatial light modulator to project 473 nm light patterns surrounding a region of adherent cells and achieved an arbitrarily micropatterned cell culture. For a group of ∼60 cells, the cell boundaries fit the pattern of light within 15% deviation of the side length. We also demonstrated a wound-healing experiment with a definite starting temporal point by using this technique. While observing mitochondrial structures in the illuminated cells, we found that the 473 nm light damaged the integrity of mitochondria and thus prohibited cell proliferation in the illuminated region.
Authors:
Jian-Long Xiao; Huei-Jyuan Pan; Chau-Hwang Lee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  17     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-08-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  075004     Citation Subset:  IM    
Affiliation:
National Yang-Ming University, Institute of Biophotonics, Taipei 11221, Taiwan.
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