Document Detail

An Optically Controlled 3D Cell Culturing System.
MedLine Citation:
PMID:  22701475     Owner:  NLM     Status:  Publisher    
A novel 3D cell culture system was developed and tested. The cell culture device consists of a microfluidic chamber on an optically absorbing substrate. Cells are suspended in a thermoresponsive hydrogel solution, and optical patterns are utilized to heat the solution, producing localized hydrogel formation around cells of interest. The hydrogel traps only the desired cells in place while also serving as a biocompatible scaffold for supporting the cultivation of cells in 3D. This is demonstrated with the trapping of MDCK II and HeLa cells. The light intensity from the optically induced hydrogel formation does not significantly affect cell viability.
Kelly S Ishii; Wenqi Hu; Swapnil A Namekar; Aaron T Ohta
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-12
Journal Detail:
Title:  Advances in optoelectronics     Volume:  2011     ISSN:  1687-5648     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2012-6-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101583714     Medline TA:  Adv Optoelectron     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Electrical Engineering, University of Hawaii at Manoa, 2540 Dole Street, Holmes Hall 483, Honolulu, HI 96822, USA.
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