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Optical trapping microrheology in cultured human cells.
MedLine Citation:
PMID:  22821510     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
We present the microrheological study of the two close human epithelial cell lines: non-cancerous HCV29 and cancerous T24. The optical tweezers tracking was applied to extract the several seconds long trajectories of endogenous lipid granules at time step of 1μs. They were analyzed using a recently proposed equation for mean square displacement (MSD) in the case of subdiffusion influenced by an optical trap. This equation leads to an explicit form for viscoelastic moduli. The moduli of the two cell lines were found to be the same within the experimental accuracy for frequencies 10(2) - 10(5) Hz. For both cell lines subdiffusion was observed with the exponent close to 3/4, the value predicted by the theory of semiflexible polymers. For times longer than 0.1s the MSD of cancerous cells exceeds the MSD of non-cancerous cells for all values of the trapping force. Such behavior can be interpreted as a signature of the active processes and prevents the extraction of the low-frequency viscoelastic moduli for the living cells by passive microrheology.
Authors:
E Bertseva; D Grebenkov; P Schmidhauser; S Gribkova; S Jeney; L Forró
Publication Detail:
Type:  Journal Article     Date:  2012-07-23
Journal Detail:
Title:  The European physical journal. E, Soft matter     Volume:  35     ISSN:  1292-895X     ISO Abbreviation:  Eur Phys J E Soft Matter     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101126530     Medline TA:  Eur Phys J E Soft Matter     Country:  France    
Other Details:
Languages:  eng     Pagination:  9739     Citation Subset:  IM    
Affiliation:
Laboratory of Physics of Complex Matter, Ecole polytechnique federale de Lausanne (EPFL), Station 3, CH-1015, Lausanne VD, Switzerland, elena.bertseva@epfl.ch.
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