Document Detail

Optical techniques for imaging membrane topography.
MedLine Citation:
PMID:  15509889     Owner:  NLM     Status:  MEDLINE    
In recent years three powerful optical imaging techniques have emerged that provide nanometer-scale information about the topography of membrane surfaces, whether cellular or artificial: intermembrane fluorescence resonance energy transfer (FRET), fluorescence interference contrast microscopy (FLIC), and reflection interference contrast microscopy (RICM). In intermembrane FRET, the sharp distance dependence of resonant energy transfer between fluorophores allows topographic measurements in the Angstrom to few-nanometer range. In FLIC and RICM, interference between light from a membrane (either from fluorescent probes, or reflected illumination) and light reflected by a planar substrate provide spatial sensitivity in the few to hundreds of nanometer range, with few-nanometer resolution. All of these techniques are fairly easy to implement. We discuss the physics and optics behind each of these tools, as well as practical concerns regarding their uses. We also provide examples of their application in imaging molecular-scale structures at intermembrane junctions.
Raghuveer Parthasarathy; Jay T Groves
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Cell biochemistry and biophysics     Volume:  41     ISSN:  1559-0283     ISO Abbreviation:  Cell Biochem. Biophys.     Publication Date:  2004  
Date Detail:
Created Date:  2004-10-28     Completed Date:  2009-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9701934     Medline TA:  Cell Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  391-414     Citation Subset:  IM    
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
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MeSH Terms
Biophysics / methods
Cell Membrane / metabolism*
Fluorescence Resonance Energy Transfer / methods*
Fluorescent Dyes / pharmacology
Lipid Bilayers / chemistry*
Microscopy, Fluorescence / methods*
Microscopy, Phase-Contrast / methods*
Models, Statistical
Optics and Photonics
Oxides / chemistry
Silicon Dioxide / chemistry
Reg. No./Substance:
0/Fluorescent Dyes; 0/Lipid Bilayers; 0/Oxides; 7631-86-9/Silicon Dioxide

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