Document Detail

Optical stimulation and imaging of functional brain circuitry in a segmented laminar flow chamber.
MedLine Citation:
PMID:  23044655     Owner:  NLM     Status:  MEDLINE    
Microfluidic technology is emerging as a useful tool for the study of brain slices, offering precise delivery of chemical factors along with robust oxygen and nutrient transport. However, continued reliance upon electrode-based physiological recording poses inherent limitations in terms of physical access, as well as the number of sites that can be sampled simultaneously. In the present study, we combine a microfluidic laminar flow chamber with fast voltage-sensitive dye imaging and laser photostimulation via caged glutamate to map neural network activity across large cortical regions in living brain slices. We find that the closed microfluidic chamber results in greatly improved signal-to-noise performance for optical measurements of neural signaling. These optical tools are also leveraged to characterize laminar flow interfaces within the device, demonstrating a functional boundary width of less than 100 μm. Finally, we utilize this integrated platform to investigate the mechanism of signal propagation for spontaneous neural activity in the developing mouse hippocampus. Through the use of localized Ca(2+) depletion, we provide evidence for Ca(2+)-dependent synaptic transmission.
Siavash Ahrar; Transon V Nguyen; Yulin Shi; Taruna Ikrar; Xiangmin Xu; Elliot E Hui
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Lab on a chip     Volume:  13     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-24     Completed Date:  2013-10-30     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  536-41     Citation Subset:  IM    
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MeSH Terms
Cerebral Cortex / cytology,  physiology*
Hippocampus / cytology,  physiology*
Microfluidic Analytical Techniques* / instrumentation
Optics and Photonics*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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