Document Detail

Optical silencing of C. elegans cells with light-driven proton pumps.
MedLine Citation:
PMID:  24593985     Owner:  NLM     Status:  Publisher    
Recent development of optogenetic techniques, which utilize light-driven ion channels or ion pumps for controlling the activity of excitable cells, has greatly facilitated the investigation of nervous systems in vivo. A new generation of optical silencers includes outward-directed proton pumps, such as Arch, which have several advantages over currently widely used halorhodopsin (NpHR). These advantages include the resistance to inactivation during prolonged illumination and the ability to generate a larger optical current from low intensity light. C. elegans, with its small transparent body and well-characterized neural circuits, is especially suitable for optogenetic analyses. In this article, we will outline the practical aspects of using of Arch and other proton pumps as optogenetic tools in C. elegans.
Ayako Okazaki; Megumi Takahashi; Naoya Toyoda; Shin Takagi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-1
Journal Detail:
Title:  Methods (San Diego, Calif.)     Volume:  -     ISSN:  1095-9130     ISO Abbreviation:  Methods     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-3-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426302     Medline TA:  Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014. Published by Elsevier Inc.
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