| Optical monitoring of microlymphatic disturbances during experimental lymphedema. | |
| | |
MedLine Citation:
|
PMID: 17508899 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Rat mesentery has been widely used to study microvascular functions. The goal of this work is to extend this animal model to monitor blood and lymph microvessel function during lymphedema. METHODS AND RESULTS: Lymphedema is created by microsurgical removal of regional lymph nodes (lymphadenectomy) or ligation of the collecting vein. Water volume in mesenteric tissue, microvessel diameters, phasic contraction, valve function, lymph flow velocity, and cell migration were analyzed during lymphedema development. Dynamic observation of water amount after lymphadenectomy revealed increasing edema from 30 min to 1 week; greatest degree of edema at one week, and gradual decrease in edema from 1 to 11 weeks. These effects were accompanied by acute constriction of lymph vessels and slowing of lymph flow velocity, switching to dilation and appearance of new blood capillaries at week 1, progressing to dilation and degenerative changes of the microlymphatic wall at week 4, and, finally, leading to lymphatic fibrosis and lymphangiogenesis at week 11. Acute venous insufficiency (30 min after vein ligation) led to significant edema, decreasing blood flow velocity to stasis, and output of erythrocytes from venules to interstitium, with further movement to microlymphatics and regional lymph nodes. CONCLUSIONS: Rat mesentery as an animal model in combination with an advanced optical imaging system is valuable in studying microlymphatic disturbances in mesentery during the development of experimental lymphedema from latent period to chronic stages, including monitoring of individual cell dislocation with high resolution optical imaging. |
| | |
Authors:
|
E I Galanzha; V V Tuchin; V P Zharov |
Related Documents
:
|
3434089 - Changes in bile and lymph composition following portacaval anastomosis (pca) in rat. 12120199 - Has blood glucose level measured on admission to hospital in a patient with acute pancr... 14599719 - Effect of exogenous cholecystokinin on islet blood flow in anesthetized rats. 830789 - The flow of blood to lymph nodes and its relation to lymphocyte traffic and the immune ... 7557149 - Pancreatic interstitial ph in human and feline chronic pancreatitis. 11005159 - Improvement of macromolecular clearance via lymph flow in hamster gingiva by topical wa... 14600279 - Dermal, oral, and inhalation pharmacokinetics of methyl tertiary butyl ether (mtbe) in ... 11599059 - Functional cardiac mr imaging with steady-state free precession (ssfp) significantly im... 18258819 - Angiopoietin-related growth factor enhances blood flow via activation of the erk1/2-eno... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Lymphatic research and biology Volume: 5 ISSN: 1539-6851 ISO Abbreviation: Lymphat Res Biol Publication Date: 2007 |
Date Detail:
|
Created Date: 2007-05-18 Completed Date: 2007-07-06 Revised Date: 2011-01-11 |
Medline Journal Info:
|
Nlm Unique ID: 101163587 Medline TA: Lymphat Res Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 11-27 Citation Subset: IM |
Affiliation:
|
Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences (UAMS), Little Rock, Arkansas 72205-7199, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Capillaries / ultrastructure* Disease Models, Animal* Imaging, Three-Dimensional* Lymphatic Vessels / ultrastructure* Lymphedema / pathology* Male Mesentery / blood supply, surgery, ultrastructure Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
|
EB000873/EB/NIBIB NIH HHS; EB001858/EB/NIBIB NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The primary valves in the initial lymphatics during inflammation.
Next Document: Spontaneous electrical activity in sheep mesenteric lymphatics.