Document Detail

Optical measurement of isolated canine lung filtration coefficients after alloxan infusion.
MedLine Citation:
PMID:  9516207     Owner:  NLM     Status:  MEDLINE    
In this study, lung filtration coefficient (Kfc) was measured in eight isolated canine lung preparations by using three methods: standard gravimetric (Std), blood-corrected gravimetric (BC), and optical. The lungs were held in zone III conditions and were subjected to an average venous pressure increase of 8.79 +/- 0.93 (mean +/- SD) cmH2O. The permeability of the lungs was increased with an infusion of alloxan (75 mg/kg). The resulting Kfc values (in milliliters . min-1 . cmH2O-1 . 100 g dry lung weight-1) measured by using Std and BC gravimetric techniques before vs. after alloxan infusion were statistically different: Std, 0.527 +/- 0.290 vs. 1. 966 +/- 0.283; BC, 0.313 +/- 0.290 vs. 1.384 +/- 0.290. However, the optical technique did not show any statistical difference between pre- and postinjury with alloxan, 0.280 +/- 0.305 vs. 0.483 +/- 0. 297, respectively. The alloxan injury, quantified by using multiple-indicator techniques, showed an increase in permeability and a corresponding decrease in reflection coefficient for albumin (sigmaf). Because the optical method measures the product of Kfc and sigmaf, this study shows that albumin should not be used as an intravascular optical filtration marker when permeability is elevated. However, the optical technique, along with another means of measuring Kfc (such as BC), can be used to calculate the sigmaf of a tracer (in this study, sigmaf of 0.894 at baseline and 0.348 after injury). Another important finding of this study was that the ratio of baseline-to-injury Kfc values was not statistically different for Std and BC techniques, indicating that the percent contribution of slow blood-volume increases does not change because of injury.
J W Klaesner; N A Pou; R E Parker; C Finney; R J Roselli
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  84     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-05-05     Completed Date:  1998-05-05     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1381-7     Citation Subset:  IM    
Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee 37235, USA.
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MeSH Terms
Alloxan / pharmacology*
Blood Pressure / drug effects,  physiology
Blood-Air Barrier / drug effects,  physiology
Coloring Agents
Evans Blue
Indicator Dilution Techniques
Lung / drug effects,  physiology*
Models, Biological
Permeability / drug effects
Respiratory Function Tests / instrumentation,  methods*
Grant Support
Reg. No./Substance:
0/Coloring Agents; 314-13-6/Evans Blue; 50-71-5/Alloxan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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