| Optical mapping study of blebbistatin-induced chaotic electrical activities in isolated rat atrium preparations. | |
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MedLine Citation:
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PMID: 20013327 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have studied the spatiotemporal pattern of blebbistatin-induced anomalous electrical activities in isolated rat atrial preparations using the optical mapping of excitation spread. Atrial preparations including the right or left auricle were dissected from adult rat hearts. Each preparation was then stained with a fast merocyanine-rhodanine voltage-sensitive dye (NK2761). Using a multi-element (16 x 16) photodiode array, we assessed the spread of excitation optically by timing the initiation of the action potential-related extrinsic absorption changes. The contraction-related optical signals were suppressed by adding (S)-(-)-blebbistatin (10-100 miocroM) to the bathing solution. Blebbistatin had an effective delay time of about 1.5 h following its application, at which time anomalous electrical activities occurred. These took the form of triggered activities and rhythmical spontaneous excitations. We optically mapped the spatiotemporal patterns of the excitation spread during these anomalous electrical activities. When the triggered activities occurred, the site of ectopic focus, where the triggered action potential first appeared, and the area of excitation spread varied in every event. When the rhythmical spontaneous excitations occurred, the excitation spread from the anomalous pacemaker and, occasionally, their spatial shift was observed. In addition, the combination pattern of the spontaneous excitations and triggered activities was also observed. We suggest that these phenomena are due to the disturbed intracellular calcium dynamics induced by the application of blebbistatin. |
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Authors:
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Natnicha Kanlop; Tetsuro Sakai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-16 |
Journal Detail:
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Title: The journal of physiological sciences : JPS Volume: 60 ISSN: 1880-6562 ISO Abbreviation: J Physiol Sci Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-05-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101262417 Medline TA: J Physiol Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 109-17 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of the Ryukyus School of Medicine, 207 Uehara, Nishihara, Okinawa, 903-0215, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials
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drug effects Animals Arrhythmias, Cardiac / chemically induced*, physiopathology* Body Surface Potential Mapping / drug effects, methods* Female Heart Atria / drug effects, physiopathology* Heart Conduction System / drug effects, physiopathology* Heterocyclic Compounds with 4 or More Rings* Male Microscopy, Fluorescence / methods Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Heterocyclic Compounds with 4 or More Rings; 0/blebbistatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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