Document Detail


Optical levitation and manipulation of stuck particles with pulsed optical tweezers.
MedLine Citation:
PMID:  16092349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report on optical levitation and manipulation of microscopic particles that are stuck on a glass surface with pulsed optical tweezers. An infrared pulse laser at 1.06 microm was used to generate a large gradient force (up to 10(-9) N) within a short duration (approximately 45 micros) that overcomes the adhesive interaction between the particles and the glass surface. Then a low-power continuous-wave diode laser at 785 nm was used to capture and manipulate the levitated particle. We have demonstrated that both stuck dielectric and biological micrometer-sized particles, including polystyrene beads, yeast cells, and Bacillus cereus bacteria, can be levitated and manipulated with this technique. We measured the single-pulse levitation efficiency for 2.0 microm polystyrene beads as a function of the pulse energy and of the axial displacement from the stuck particle to the pulsed laser focus, which was as high as 88%.
Authors:
Amol Ashok Ambardekar; Yong-Qing Li
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Optics letters     Volume:  30     ISSN:  0146-9592     ISO Abbreviation:  Opt Lett     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-08-11     Completed Date:  2005-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7708433     Medline TA:  Opt Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1797-9     Citation Subset:  IM    
Affiliation:
Department of Physics, East Carolina University, Greenville, North Carolina 27858-4353, USA.
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MeSH Terms
Descriptor/Qualifier:
Adhesiveness
Bacillus cereus / cytology*,  physiology*
Cell Adhesion
Cell Movement / physiology
Cell Separation / instrumentation*,  methods
Equipment Design
Equipment Failure Analysis
Lasers*
Micromanipulation / instrumentation*,  methods
Microspheres
Particle Size
Physical Stimulation / methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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