Document Detail

Optical fluorescent imaging to monitor temporal effects of microbubble-mediated ultrasound therapy.
MedLine Citation:
PMID:  23357902     Owner:  NLM     Status:  MEDLINE    
Microbubble-mediated ultrasound therapy can noninvasively enhance drug delivery to localized regions in the body. This technique can be beneficial in cancer therapy, but currently there are limitations to tracking the therapeutic effects. The purpose of this experiment was to investigate the potential of fluorescent imaging for monitoring the temporal effects of microbubble-mediated ultrasound therapy. Mice were implanted with 2LMP breast cancer cells. The animals underwent microbubble-mediated ultrasound therapy in the presence of Cy5.5 fluorescent-labeled IgG antibody (large molecule) or Cy5.5 dye (small molecule) and microbubble contrast agents. Control animals were administered fluorescent molecules only. Animals were transiently imaged in vivo at 1, 10, 30, and 60 min post therapy using a small animal optical imaging system. Tumors were excised and analyzed ex vivo. Tumors were homogenized and emulsion imaged for Cy5.5 fluorescence. Monitoring in vivo results showed significant influx of dye into the tumor (p < 0.05) using the small molecule, but not in the large molecule group (p > 0.05). However, after tumor emulsion, significantly higher dye concentration was detected in therapy group tumors for both small and large molecule groups in comparison to their control counterparts (p <0.01). This paper explores a noninvasive optical imaging method for monitoring the effects of microbubble-mediated ultrasound therapy in a cancer model. It provides temporal information following the process of increasing extravasation of molecules into target tumors.
Anna G Sorace; Reshu Saini; Eben Rosenthal; Jason M Warram; Kurt R Zinn; Kenneth Hoyt
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  IEEE transactions on ultrasonics, ferroelectrics, and frequency control     Volume:  60     ISSN:  1525-8955     ISO Abbreviation:  IEEE Trans Ultrason Ferroelectr Freq Control     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-29     Completed Date:  2013-07-08     Revised Date:  2014-01-14    
Medline Journal Info:
Nlm Unique ID:  9882735     Medline TA:  IEEE Trans Ultrason Ferroelectr Freq Control     Country:  United States    
Other Details:
Languages:  eng     Pagination:  281-9     Citation Subset:  IM    
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MeSH Terms
Carbocyanines / chemistry,  pharmacokinetics
Cell Line, Tumor
Contrast Media / chemistry,  pharmacokinetics
Fluorescent Dyes / chemistry,  pharmacokinetics
Immunoglobulin G / metabolism
Mammary Neoplasms, Experimental / metabolism
Mice, Nude
Optical Imaging / methods*
Ultrasonic Therapy / methods*
Grant Support
Reg. No./Substance:
0/CY5.5 cyanine dye; 0/Carbocyanines; 0/Contrast Media; 0/Fluorescent Dyes; 0/Immunoglobulin G

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