Document Detail


Optical fiber light-emitting diode-induced fluorescence detection for capillary electrophoresis.
MedLine Citation:
PMID:  16358357     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A highly sensitive optical fiber light-emitting diode (LED)-induced fluorescence detector for CE has been constructed and evaluated. In this detector, a violet or blue LED was used as the excitation source and an optical fiber with 40 microm OD was used to transmit the excitation light. The upper end of the fiber was inserted into the separation capillary and was situated right at the detection window. Fluorescence emission was collected by a 40 x microscope objective, focused on a spatial filter, and passed through a cutoff filter before reaching the photomultiplier tube. Output signals were recorded and processed with a computer using in-house written software. The present CE/fluorescence detector deploys a simple and inexpensive optical system that requires only an LED as the light source. Its utility was successfully demonstrated by the separation and determination of amino acids (AAs) labeled with naphthalene-2,3-dicarboxaldehyde (NDA) and FITC. Low detection limits were obtained ranging from 17 to 23 nM for NDA-tagged AAs and 8 to 12 nM for FITC-labeled AAs (S/N=3). By virtue of such valuable features as low cost, convenience, and miniaturization, the presented detection scheme was proven to be attractive for sensitive fluorescence detection in CE.
Authors:
Shulin Zhao; Hongyan Yuan; Dan Xiao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  27     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-01     Completed Date:  2006-03-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  461-7     Citation Subset:  IM    
Affiliation:
College of Chemical Engineering, Sichuan University, Chengdu, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Electrophoresis, Capillary / instrumentation*
Fluorescein-5-isothiocyanate / chemistry
Fluorometry
Light*
Naphthalenes / chemistry
Optics and Photonics
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Naphthalenes; 3326-32-7/Fluorescein-5-isothiocyanate; 7149-49-7/2,3-naphthalenedicarboxaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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