Document Detail

Optical and electrochemical detection of saccharides with poly(aniline-co-3-aminobenzeneboronic acid) prepared from enzymatic polymerization.
MedLine Citation:
PMID:  17918994     Owner:  NLM     Status:  MEDLINE    
Boronic acid-based sensors for saccharides have been developed via biocatalysis. The self-doped copolymer of poly(aniline-co-3-aminobenzeneboronic acid) [poly(aniline-co-AB)], with various mole ratios of two components, was synthesized by oxidative enzymatic polymerization using a natural biocatalyst such as horseradish peroxidase together with an anionic polyelectrolyte template (sulfonated polystyrene) under mild conditions (pH 4.5). Poly(aniline-co-AB), having an aniline boronic acid-to-aniline ratio of 1:2 on average, gave rise to a green doped polymer with absorption maxima at 745 nm. The potentiometric detection of saccharides using poly(aniline-co-AB) is presented. Characteristics of both transient and steady-state response associated with the complex formation of poly(aniline-co-AB) with various saccharides were monitored by UV-vis spectroscopy and cyclic voltammetry (CV). The results obtained from UV-vis spectroscopy and CV show that the sensitivity of enzymatically synthesized water-soluble poly(aniline-co-AB) for various saccharides was improved significantly compared to the chemically synthesized counterpart. A possible mechanism for the sensitive detection of sugar molecules by boronic acid is proposed on the basis of UV-vis and IR spectrophotometry, and four-point probe conductivity measurements.
Pilho Huh; Seong-Cheol Kim; Younghoon Kim; Yanping Wang; Jagdeep Singh; Jayant Kumar; Lynne A Samuelson; Bong-Soo Kim; Nam-Ju Jo; Jang-Oo Lee
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-10-06
Journal Detail:
Title:  Biomacromolecules     Volume:  8     ISSN:  1525-7797     ISO Abbreviation:  Biomacromolecules     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-11-12     Completed Date:  2007-12-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3602-7     Citation Subset:  IM    
Center for Advanced Materials, Polymer Science Program, Departments of Chemistry and Physics, University of Massachusetts, Lowell, MA 01854,USA.
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MeSH Terms
Aniline Compounds / chemistry*,  metabolism*
Boronic Acids / chemistry*,  metabolism*
Carbohydrate Metabolism*
Carbohydrates / chemistry*
Horseradish Peroxidase / metabolism*
Molecular Structure
Reg. No./Substance:
0/Aniline Compounds; 0/Boronic Acids; 0/Carbohydrates; 0/polyaniline; 30418-59-8/3-aminobenzeneboronic acid; EC 1.11.1.-/Horseradish Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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