| Optical and electrochemical detection of saccharides with poly(aniline-co-3-aminobenzeneboronic acid) prepared from enzymatic polymerization. | |
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MedLine Citation:
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PMID: 17918994 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Boronic acid-based sensors for saccharides have been developed via biocatalysis. The self-doped copolymer of poly(aniline-co-3-aminobenzeneboronic acid) [poly(aniline-co-AB)], with various mole ratios of two components, was synthesized by oxidative enzymatic polymerization using a natural biocatalyst such as horseradish peroxidase together with an anionic polyelectrolyte template (sulfonated polystyrene) under mild conditions (pH 4.5). Poly(aniline-co-AB), having an aniline boronic acid-to-aniline ratio of 1:2 on average, gave rise to a green doped polymer with absorption maxima at 745 nm. The potentiometric detection of saccharides using poly(aniline-co-AB) is presented. Characteristics of both transient and steady-state response associated with the complex formation of poly(aniline-co-AB) with various saccharides were monitored by UV-vis spectroscopy and cyclic voltammetry (CV). The results obtained from UV-vis spectroscopy and CV show that the sensitivity of enzymatically synthesized water-soluble poly(aniline-co-AB) for various saccharides was improved significantly compared to the chemically synthesized counterpart. A possible mechanism for the sensitive detection of sugar molecules by boronic acid is proposed on the basis of UV-vis and IR spectrophotometry, and four-point probe conductivity measurements. |
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Authors:
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Pilho Huh; Seong-Cheol Kim; Younghoon Kim; Yanping Wang; Jagdeep Singh; Jayant Kumar; Lynne A Samuelson; Bong-Soo Kim; Nam-Ju Jo; Jang-Oo Lee |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2007-10-06 |
Journal Detail:
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Title: Biomacromolecules Volume: 8 ISSN: 1525-7797 ISO Abbreviation: Biomacromolecules Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-12 Completed Date: 2007-12-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 3602-7 Citation Subset: IM |
Affiliation:
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Center for Advanced Materials, Polymer Science Program, Departments of Chemistry and Physics, University of Massachusetts, Lowell, MA 01854,USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aniline Compounds
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chemistry*,
metabolism* Boronic Acids / chemistry*, metabolism* Carbohydrate Metabolism* Carbohydrates / chemistry* Electrochemistry Horseradish Peroxidase / metabolism* Molecular Structure Spectrophotometry |
| Chemical | |
Reg. No./Substance:
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0/Aniline Compounds; 0/Boronic Acids; 0/Carbohydrates; 0/polyaniline; 30418-59-8/3-aminobenzeneboronic acid; EC 1.11.1.-/Horseradish Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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