Document Detail


Optical coherence tomography captures rapid hemodynamic responses to acute hypoxia in the cardiovascular system of early embryos.
MedLine Citation:
PMID:  22275053     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The trajectory to heart defects may start in tubular and looping heart stages when detailed analysis of form and function is difficult by currently available methods. We used a novel method, Doppler optical coherence tomography (OCT), to follow changes in cardiovascular function in quail embryos during acute hypoxic stress. Chronic fetal hypoxia is a known risk factor for congenital heart diseases (CHDs). Decreased fetal heart rates during maternal obstructive sleep apnea suggest that studying fetal heart responses under acute hypoxia is warranted.
RESULTS: We captured responses to hypoxia at the critical looping heart stages. Doppler OCT revealed detailed vitelline arterial pulsed Doppler waveforms. Embryos tolerated 1 hr of hypoxia (5%, 10%, or 15% O(2) ), but exhibited changes including decreased systolic and increased diastolic duration in 5 min. After 5 min, slower heart rates, arrhythmic events and an increase in retrograde blood flow were observed. These changes suggested slower filling of the heart, which was confirmed by four-dimensional Doppler imaging of the heart itself.
CONCLUSIONS: Doppler OCT is well suited for rapid noninvasive screening for functional changes in avian embryos under near physiological conditions. Analysis of the accessible vitelline artery sensitively reflected changes in heart function and can be used for rapid screening. Acute hypoxia caused rapid hemodynamic changes in looping hearts and may be a concern for increased CHD risk.
Authors:
Shi Gu; Michael W Jenkins; Lindsy M Peterson; Yong-Qiu Doughman; Andrew M Rollins; Michiko Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, American Recovery and Reinvestment Act; Research Support, N.I.H., Extramural     Date:  2012-01-23
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  241     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-22     Completed Date:  2012-07-20     Revised Date:  2014-04-21    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  534-44     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cardiovascular System / physiopathology*
Embryo, Nonmammalian / blood supply*,  physiopathology*
Fetal Hypoxia / physiopathology*
Heart Defects, Congenital / embryology*,  physiopathology
Heart Rate
Hemodynamics*
Quail
Tomography, Optical Coherence
Grant Support
ID/Acronym/Agency:
HL083048/HL/NHLBI NIH HHS; HL091171/HL/NHLBI NIH HHS; HL095717/HL/NHLBI NIH HHS; R01 HL083048/HL/NHLBI NIH HHS; R01 HL083048-01/HL/NHLBI NIH HHS; R01 HL083048-02/HL/NHLBI NIH HHS; R01 HL083048-03/HL/NHLBI NIH HHS; R01 HL083048-04/HL/NHLBI NIH HHS; R01 HL091171/HL/NHLBI NIH HHS; R01 HL091171-01A1/HL/NHLBI NIH HHS; R01 HL091171-02/HL/NHLBI NIH HHS; R01 HL095717/HL/NHLBI NIH HHS; R01 HL095717-01/HL/NHLBI NIH HHS; R01 HL095717-02/HL/NHLBI NIH HHS; R01 HL095717-03/HL/NHLBI NIH HHS; T32 EB007509/EB/NIBIB NIH HHS
Comments/Corrections

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