| Optical coherence tomography as a rapid, accurate, noncontact method of visualizing the palisades of Vogt. | |
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MedLine Citation:
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PMID: 22266521 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: This study explored the efficacy of optical coherence tomography (OCT) as a high-resolution, noncontact method for imaging the palisades of Vogt by correlating OCT and confocal microscopy images. METHODS: Human limbal rims were acquired and imaged with OCT and confocal microscopy. The area of the epithelial basement membrane in each of these sets was digitally reconstructed, and the models were compared. RESULTS: OCT identified the palisades within the limbus and exhibited excellent structural correlation with immunostained tissue imaged by confocal microscopy. CONCLUSIONS: OCT successfully identified the limbal palisades of Vogt that constitute the corneal epithelial stem cell niche. These findings offer the exciting potential to characterize the architecture of the palisades in vivo, to harvest stem cells for transplantation more accurately, to track palisade structure for better diagnosis, follow-up and staging of treatment, and to assess and intervene in the progression of stem cell depletion by monitoring changes in the structure of the palisades. |
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Authors:
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Kira L Lathrop; Divya Gupta; Larry Kagemann; Joel S Schuman; Nirmala Sundarraj |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-03-15 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 53 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-03-19 Completed Date: 2012-05-03 Revised Date: 2013-06-11 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 1381-7 Citation Subset: IM |
Affiliation:
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University of Pittsburgh School of Medicine, Department of Ophthalmology, Eye and Ear Institute, Room 1026, 203 Lothrop Street, Pittsburgh, PA 15213, USA. lathropkl@upmc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Epithelium, Corneal / anatomy & histology* Female Humans Limbus Corneae / anatomy & histology* Male Microscopy, Confocal / methods Middle Aged Reference Values Tomography, Optical Coherence / methods* Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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EY03263/EY/NEI NIH HHS; EY08090/EY/NEI NIH HHS; R01 EY013178/EY/NEI NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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