Document Detail


Optical coding of mammalian cells using semiconductor quantum dots.
MedLine Citation:
PMID:  15051536     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell-based assays are widely used to screen compounds and study complex phenotypes. Few methods exist, however, for multiplexing cellular assays or labeling individual cells in a mixed cell population. We developed a generic encoding method for cells that is based on peptide-mediated delivery of quantum dots (QDs) into live cells. The QDs are nontoxic and photostable and can be imaged using conventional fluorescence microscopy or flow cytometry systems. We created unique fluorescent codes for a variety of mammalian cell types and show that our encoding method has the potential to create > 100 codes. We demonstrate that QD cell codes are compatible with most types of compound screening assays including immunostaining, competition binding, reporter gene, receptor internalization, and intracellular calcium release. A multiplexed calcium assay for G-protein-coupled receptors using QDs is demonstrated. The ability to spectrally encode individual cells with unique fluorescent barcodes should open new opportunities in multiplexed assay development and greatly facilitate the study of cell/cell interactions and other complex phenotypes in mixed cell populations.
Authors:
Larry C Mattheakis; Jennifer M Dias; Yun-Jung Choi; Jing Gong; Marcel P Bruchez; Jianquan Liu; Eugene Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  327     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-30     Completed Date:  2004-11-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  200-8     Citation Subset:  IM    
Affiliation:
Quantum Dot Corp., 26118 Research Road, Hayward, CA 94545, USA. lmattheakis@cytokinetics.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay / methods*
CHO Cells
Calcium / metabolism
Cricetinae
Cricetulus
Cysteamine / analogs & derivatives
Drug Evaluation, Preclinical / methods*
Flow Cytometry
Isoproterenol / pharmacology
Microscopy, Fluorescence*
Oligopeptides / chemistry,  metabolism
Peptides
Protein Sorting Signals
Quantum Dots*
Receptor, Muscarinic M1 / metabolism
Receptor, Serotonin, 5-HT2A / metabolism
Receptor, Serotonin, 5-HT2B / metabolism
Receptors, G-Protein-Coupled / metabolism
Semiconductors
Chemical
Reg. No./Substance:
0/Oligopeptides; 0/Pep-1 peptide; 0/Peptides; 0/Protein Sorting Signals; 0/Receptor, Muscarinic M1; 0/Receptor, Serotonin, 5-HT2A; 0/Receptor, Serotonin, 5-HT2B; 0/Receptors, G-Protein-Coupled; 60-23-1/Cysteamine; 7440-70-2/Calcium; 7683-59-2/Isoproterenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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