Document Detail


Optical analysis of perfusion bioreactor cell concentration in an acoustic separator.
MedLine Citation:
PMID:  16155953     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Automated monitoring of cell concentration in perfusion bioprocesses facilitates the maintenance of constant cell specific perfusion rates. However, most on-line measuring devices are relatively complex and foul as the culture progresses. A simple external optical sensor was developed using the transparent glass walls of acoustic separators for automated optical analysis of their contents. For each measurement, the separator was filled by an automated pumping system with triplicate representative bioreactor samples that were optically analyzed and the device returned to perfusion operation within approximately 1 or 2 min. Chinese hamster ovary cell concentrations, ranging from 5 x 10(5) to 2 x 10(7) cells/mL, were highly correlated (R(2) = 0.99) with the 90 degrees scattered light response. Since the device was operated externally, it did not complicate bioreactor sterilization or cleaning. Viability was not optically analyzed, but this information was not required between manual samples of a properly operated perfusion process. Using single-point recalibration based on routine off-line samples, this external optical system remained effective during a 4-month perfusion run, thus providing a non-invasive and easily maintained on-line cell concentration monitoring system to improve the control of perfusion bioreactors.
Authors:
Volker M Gorenflo; Vincent S Chow; Christopher Chou; James M Piret
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  92     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-10-24     Completed Date:  2005-12-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  514-8     Citation Subset:  IM    
Affiliation:
Michael Smith Laboratories, 2185 East Mall, University of British Columbia, Vancouver, British Columbia, Canada. volkergorenflo@web.de
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MeSH Terms
Descriptor/Qualifier:
Acoustics / instrumentation
Animals
Bioreactors*
CHO Cells
Cell Count* / instrumentation,  methods
Cell Separation / methods
Cricetinae
Cricetulus
Optics and Photonics / instrumentation

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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