| Optical Studies of the interaction of 4'-6'-diamidino-2-phenylindole with DNA and metaphase chromosomes. | |
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MedLine Citation:
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PMID: 67012 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The optical absorption and fluorescence characteristics of 4'-6-diamidino-2-phenylindole (DAPI) with DNA and chromosomes were studied. There is a decrease in extinction coefficient and chift in the absorption spectra to a higher wavelength when the dye binds to DNA. The fluorescence of DAPI is enhanced by both A-T and G-C base-pairs. The enhancement by A-T rich is significantly greater than by G-C rich DNA. The chromosomes and the constrictions of human chromosomes 1 and 16; these regions are known to contain A-T rich DNA and show dull fluorescence when treated with quinacrine. This dye may be useful for identifying A-T rich region in chromosomes. The fluorescence of DAPI bound to polynucleotides or chromosomes is partially quenched by the introduction of BrdU. This suppression of dye fluorescence allows optical detection of sister chromatid exchanges and chromosome region containing DNA with an unequal distribution of thymidine between polynucleotide chains after BrdU incorporation. |
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Authors:
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M S Lin; D E Comings; O S Alfi |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Chromosoma Volume: 60 ISSN: 0009-5915 ISO Abbreviation: Chromosoma Publication Date: 1977 Mar |
Date Detail:
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Created Date: 1977-06-30 Completed Date: 1977-06-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985138R Medline TA: Chromosoma Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 15-25 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine Nucleotides
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analysis Amidines Animals Binding Sites Bromodeoxyuridine Chromosomes / analysis* DNA / analysis* Fluorescent Dyes* Humans Hydrogen-Ion Concentration Indoles Mice Mitosis Polynucleotides / analysis Spectrometry, Fluorescence Staining and Labeling Thymine Nucleotides / analysis |
| Chemical | |
Reg. No./Substance:
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0/Adenine Nucleotides; 0/Amidines; 0/Fluorescent Dyes; 0/Indoles; 0/Polynucleotides; 0/Thymine Nucleotides; 59-14-3/Bromodeoxyuridine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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