Document Detail

Optical Studies of the interaction of 4'-6'-diamidino-2-phenylindole with DNA and metaphase chromosomes.
MedLine Citation:
PMID:  67012     Owner:  NLM     Status:  MEDLINE    
The optical absorption and fluorescence characteristics of 4'-6-diamidino-2-phenylindole (DAPI) with DNA and chromosomes were studied. There is a decrease in extinction coefficient and chift in the absorption spectra to a higher wavelength when the dye binds to DNA. The fluorescence of DAPI is enhanced by both A-T and G-C base-pairs. The enhancement by A-T rich is significantly greater than by G-C rich DNA. The chromosomes and the constrictions of human chromosomes 1 and 16; these regions are known to contain A-T rich DNA and show dull fluorescence when treated with quinacrine. This dye may be useful for identifying A-T rich region in chromosomes. The fluorescence of DAPI bound to polynucleotides or chromosomes is partially quenched by the introduction of BrdU. This suppression of dye fluorescence allows optical detection of sister chromatid exchanges and chromosome region containing DNA with an unequal distribution of thymidine between polynucleotide chains after BrdU incorporation.
M S Lin; D E Comings; O S Alfi
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chromosoma     Volume:  60     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-06-30     Completed Date:  1977-06-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  15-25     Citation Subset:  IM    
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MeSH Terms
Adenine Nucleotides / analysis
Binding Sites
Chromosomes / analysis*
DNA / analysis*
Fluorescent Dyes*
Hydrogen-Ion Concentration
Polynucleotides / analysis
Spectrometry, Fluorescence
Staining and Labeling
Thymine Nucleotides / analysis
Reg. No./Substance:
0/Adenine Nucleotides; 0/Amidines; 0/Fluorescent Dyes; 0/Indoles; 0/Polynucleotides; 0/Thymine Nucleotides; 59-14-3/Bromodeoxyuridine; 9007-49-2/DNA

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