Document Detail

Optical Spectroscopic Exploration of Binding of Cochineal Red-A with Two Homologous Serum Albumins.
MedLine Citation:
PMID:  22397587     Owner:  NLM     Status:  Publisher    
Cochineal Red-A is a, negatively charged, synthetic azo food colorant and a potential carcinogen. We present here the study of binding of Cochineal Red-A with two homologous serum albumins (SA), HSA and BSA, in aqueous pH 7.4 buffer using optical spectroscopic techniques. Protein intrinsic fluorescence quenching by Cochineal Red-A occurs through ground-state static interaction and its binding with BSA is stronger than with HSA. The magnitudes of thermodynamic parameters suggest that dye binding occurs principally via electrostatic complexation. Site-marker competitive binding shows that Cochineal Red-A binds primarily to site-I of SAs. CD spectra indicate that dye binding results in some conformational modification of SAs. Increase of ionic strength of medium results in lowering of binding. This study provides an important insight into possible means of removal of dye toxicity.
Priyanka Bolel; Niharendu Mahapatra; Mintu Halder
Related Documents :
12056907 - Specificity of soluble phospholipid binding sites on human factor xa.
16849337 - Membrane association of the cycling peroxisome import receptor pex5p.
21778237 - Commd1 (copper metabolism murr1 domain-containing protein 1) regulates cullin ring liga...
22666337 - Conserving critical sites for biodiversity provides disproportionate benefits to people.
21339697 - Phosphatidylinositol phosphates directly bind to neurofilament light chain (nf-l) for t...
8810297 - Ca2+ binding to the first epidermal growth factor-like domain of human blood coagulatio...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-7
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Quantitative changes in intracellular calcium and extracellular-regulated kinase activation measured...
Next Document:  Recent advances in the therapeutic applications of pyrazolines.