Document Detail

Opsonization of polyethylene wear particles regulates macrophage and osteoblast responses in vitro.
MedLine Citation:
PMID:  15459898     Owner:  NLM     Status:  MEDLINE    
The cellular reaction to wear debris may result in the failure of an artificial joint's fixation to the skeleton. The influence of debris opsinization on cell activity has received little attention. This study seeks to establish whether different proteinaceous culture environments may invoke variant cellular responses to debris challenge. Consideration of the zeta potential of a low density polyethylene particle group and an ex vitro ultrahigh molecular weight polyethylene particle group revealed that the nature of the protein adsorbants is related to the concentration of the proteins in solution. Furthermore, the composition of the adsorbed layer was shown to vary with the spectra of proteins in solution. In standard cell culture conditions zeta potential approached zero, indicating the high probability of particle agglomeration. Cell challenge studies with U937 macrophages showed that BSA and FCS protein adsorption mediated increased cell adhesion, while bovine IgG showed little change over control values. No changes in behavior of osteoblastic cells were observed in similar experiments.
A P D Elfick; S M Green; A W McCaskie; M A Birch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part B, Applied biomaterials     Volume:  71     ISSN:  1552-4973     ISO Abbreviation:  J. Biomed. Mater. Res. Part B Appl. Biomater.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-10-25     Completed Date:  2005-04-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101234238     Medline TA:  J Biomed Mater Res B Appl Biomater     Country:  United States    
Other Details:
Languages:  eng     Pagination:  244-51     Citation Subset:  IM    
Copyright Information:
(c) 2004 Wiley Periodicals, Inc.
School of Surgical & Reproductive Sciences, Trauma & Orthopaedics Group, The Medical School, University of Newcastle, Newcastle upon Tyne, NE2 4HH, UK.
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MeSH Terms
Biocompatible Materials / pharmacology*
Cell Line, Tumor
Colloids / pharmacology
Culture Media
Electromagnetic Fields
Macrophages / drug effects*
Osteoblasts / drug effects*
Particle Size
Polyethylenes / pharmacology*
Proteins / chemistry
Serum Albumin, Bovine / chemistry
U937 Cells
Reg. No./Substance:
0/Biocompatible Materials; 0/Colloids; 0/Culture Media; 0/Polyethylenes; 0/Proteins; 0/Serum Albumin, Bovine

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